Polymerase chain reaction (PCR) versus bacterial culture in detection of organisms in otitis media with effusion (OME) in children
المؤلفون المشاركون
Amin, Samih
Hamad, Mustafa
Muhyi, Mervat
Ali, Balegh
المصدر
Egyptian Journal of Ear, Nose, Throat and Allied Sciences
العدد
المجلد 10، العدد 2 (31 ديسمبر/كانون الأول 2009)، ص ص. 47-49، 3ص.
الناشر
الجمعية المصرية للأذن و الأنف و الحنجرة و العلوم المشتركة
تاريخ النشر
2009-12-31
دولة النشر
مصر
عدد الصفحات
3
التخصصات الرئيسية
الموضوعات
الملخص EN
OBGECTIVE:The aim of this prospective study was to compare between Polymerase Chain Reaction(PCR) and bacterial culture in detection of Streptococcus Pneumonia and M.
Catarrhalis in otitis media with effusion(OME) in children.
METHODS:Fifty patients having OME were included in this study between 2003-2008.
Myringotomy and tympanostomy tube insertion Was done in every patient and the middle ear effusion samples were aspirated.
The samples were subjected to bacteriological study in The form of culture and molecular study in the form of PCR using JM201 / 202-204 primer probe set for both S.pneumonia and M.catarrhalis.
Results: Bacterial cultures: Five cases(10%) were culture positive for S.pneumonia.
Six cases (12%) were culture positive for M.catarrhalis .Only one case(2%) was positive for both S.pneumonia and M.catarrhalis.
PCR testing: 18 cases (36%) were PCR positive for S.pneumonia, 22 cases(44%) were positive for M.catarrhalis, 6 cases(12%) were positive for both organism and 4 cases(8%) were negative by PCR.
The difference between the proportion of culture positive and PCR positive specimens for both organisms individually And collectively was significant (P<0.001).
CONCLUSIONS: PCR is more accurate than bacterial culture in detection of organisms in middle ear fluid in OME and that M.catarrhalis has more rule in otitis media with effusion as it is more commonly identified by PCR.:The aim of this prospective study was to compare between Polymerase Chain Reaction (PCR) and bacterial culture in detection of Streptococcus Pneumonia and M.
Catarrhalis in otitis media with effusion(OME) in children.
METHODS:Fifty patients having OME were included in this study between 2003-2008.
Myringotomy and tympanostomy tube insertion was done in every patient and the middle ear effusion samples were aspirated.
The samples were subjected to bacteriological study in The form of culture and molecular study in the form of PCR using JM201/202-204 primer probe set for both S.pneumonia and M.
catarrhalis.
Results: Bacterial cultures : Five cases(10%) were culture positive for S.pneumonia.
Six cases (12%) were culture positive for M.catarrhalis .Only one case (2%) was positive for both S.pneumonia and M.catarrhalis.
PCR testing: 18 cases (36%) were PCR positive For S.pneumonia, 22 cases (44%) were positive for M.catarrhalis, 6 cases (12%) were positive for both organism and 4 cases (8%) were Negative by PCR.
The difference between the proportion of culture positive and PCR positive specimens for both organisms individually And collectively was significant (P<0.001).
CONCLUSIONS:PCR is more accurate than bacterial culture in detection of organisms in middle ear fluid in OME and that M.catarrhalis has more rule in otitis media with effusion as it is more commonly identified by PCR.:The aim of this prospective study was to compare between Polymerase Chain Reaction (PCR) and bacterial culture in detection of Streptococcus Pneumonia and M.
Catarrhalis in otitis media with effusion (OME) in children.
METHODS:Fifty patients having OME were included in this study between 2003-2008.
Myringotomy and tympanostomy tube insertion Was done in every patient and the middle ear effusion samples were aspirated.
The samples were subjected to bacteriological study in The form of culture and molecular study in the form of PCR using JM201/202-204 primer probe set for both S.pneumonia and M.catarrhalis.
Results: Bacterial cultures: Five cases (10%) were culture positive for S.pneumonia.
Six cases (12%) were culture positive for M.catarrhalis .Only one case (2%) was positive for both S.pneumonia and M.catarrhalis.
PCR testing: 18 cases (36%) were PCR positive for S.pneumonia, 22 cases (44%) were positive for M.catarrhalis, 6 cases (12%) were positive for both organism and 4 cases (8%) were Negative by PCR.
The difference between the proportion of culture positive and PCR positive specimens for both organisms individu
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ali, Balegh& Hamad, Mustafa& Muhyi, Mervat& Amin, Samih. 2009. Polymerase chain reaction (PCR) versus bacterial culture in detection of organisms in otitis media with effusion (OME) in children. Egyptian Journal of Ear, Nose, Throat and Allied Sciences،Vol. 10, no. 2, pp.47-49.
https://search.emarefa.net/detail/BIM-94589
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ali, Balegh…[et al.]. Polymerase chain reaction (PCR) versus bacterial culture in detection of organisms in otitis media with effusion (OME) in children. Egyptian Journal of Ear, Nose, Throat and Allied Sciences Vol. 10, no. 2 (Dec. 2009), pp.47-49.
https://search.emarefa.net/detail/BIM-94589
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ali, Balegh& Hamad, Mustafa& Muhyi, Mervat& Amin, Samih. Polymerase chain reaction (PCR) versus bacterial culture in detection of organisms in otitis media with effusion (OME) in children. Egyptian Journal of Ear, Nose, Throat and Allied Sciences. 2009. Vol. 10, no. 2, pp.47-49.
https://search.emarefa.net/detail/BIM-94589
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 49
رقم السجل
BIM-94589
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر