Polymerase chain reaction (PCR)‎ versus bacterial culture in detection of organisms in otitis media with effusion (OME)‎ in children

Abstract EN

OBGECTIVE:The aim of this prospective study was to compare between Polymerase Chain Reaction(PCR) and bacterial culture in detection of Streptococcus Pneumonia and M.

Catarrhalis in otitis media with effusion(OME) in children.

METHODS:Fifty patients having OME were included in this study between 2003-2008.

Myringotomy and tympanostomy tube insertion Was done in every patient and the middle ear effusion samples were aspirated.

The samples were subjected to bacteriological study in The form of culture and molecular study in the form of PCR using JM201 / 202-204 primer probe set for both S.pneumonia and M.catarrhalis.

Results: Bacterial cultures: Five cases(10%) were culture positive for S.pneumonia.

Six cases (12%) were culture positive for M.catarrhalis .Only one case(2%) was positive for both S.pneumonia and M.catarrhalis.

PCR testing: 18 cases (36%) were PCR positive for S.pneumonia, 22 cases(44%) were positive for M.catarrhalis, 6 cases(12%) were positive for both organism and 4 cases(8%) were negative by PCR.

The difference between the proportion of culture positive and PCR positive specimens for both organisms individually And collectively was significant (P<0.001).

CONCLUSIONS: PCR is more accurate than bacterial culture in detection of organisms in middle ear fluid in OME and that M.catarrhalis has more rule in otitis media with effusion as it is more commonly identified by PCR.:The aim of this prospective study was to compare between Polymerase Chain Reaction (PCR) and bacterial culture in detection of Streptococcus Pneumonia and M.

Catarrhalis in otitis media with effusion(OME) in children.

METHODS:Fifty patients having OME were included in this study between 2003-2008.

Myringotomy and tympanostomy tube insertion was done in every patient and the middle ear effusion samples were aspirated.

The samples were subjected to bacteriological study in The form of culture and molecular study in the form of PCR using JM201/202-204 primer probe set for both S.pneumonia and M.

catarrhalis.

Results: Bacterial cultures : Five cases(10%) were culture positive for S.pneumonia.

Six cases (12%) were culture positive for M.catarrhalis .Only one case (2%) was positive for both S.pneumonia and M.catarrhalis.

PCR testing: 18 cases (36%) were PCR positive For S.pneumonia, 22 cases (44%) were positive for M.catarrhalis, 6 cases (12%) were positive for both organism and 4 cases (8%) were Negative by PCR.

The difference between the proportion of culture positive and PCR positive specimens for both organisms individually And collectively was significant (P<0.001).

CONCLUSIONS:PCR is more accurate than bacterial culture in detection of organisms in middle ear fluid in OME and that M.catarrhalis has more rule in otitis media with effusion as it is more commonly identified by PCR.:The aim of this prospective study was to compare between Polymerase Chain Reaction (PCR) and bacterial culture in detection of Streptococcus Pneumonia and M.

Catarrhalis in otitis media with effusion (OME) in children.

METHODS:Fifty patients having OME were included in this study between 2003-2008.

Myringotomy and tympanostomy tube insertion Was done in every patient and the middle ear effusion samples were aspirated.

The samples were subjected to bacteriological study in The form of culture and molecular study in the form of PCR using JM201/202-204 primer probe set for both S.pneumonia and M.catarrhalis.

Results: Bacterial cultures: Five cases (10%) were culture positive for S.pneumonia.

Six cases (12%) were culture positive for M.catarrhalis .Only one case (2%) was positive for both S.pneumonia and M.catarrhalis.

PCR testing: 18 cases (36%) were PCR positive for S.pneumonia, 22 cases (44%) were positive for M.catarrhalis, 6 cases (12%) were positive for both organism and 4 cases (8%) were Negative by PCR.

The difference between the proportion of culture positive and PCR positive specimens for both organisms individu