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Extraction and characterization of Arylesterase enzyme from (peel and pulp) of Annona muricata fruit
العناوين الأخرى
استخلاص و توصيف انزيم اريل استيريز من لب و قشره ثمره القشطه
المؤلفون المشاركون
Almashhdani, Firdaws A.
Bakr, Lama Abd al-Munim
Abd Allah, Sayrawan Ahmad
المصدر
Kirkuk University Journal-Scientific Studies
العدد
المجلد 12، العدد 2 (31 مارس/آذار 2017)24ص.
الناشر
تاريخ النشر
2017-03-31
دولة النشر
العراق
عدد الصفحات
24
التخصصات الرئيسية
الملخص EN
The extraction of arylesterase from the aqueous extract of (peel and pulp) of Annona muricata fruit was conducted using different biochemical techniques.
It was shown that, max activity was obtained in the pulp than in peel, and by using gel filtration chromatography on sephadex G-75 for the pulp part, the solution of the proteinous precipitate produced by acetone precipitation, contained three proteinous peaks.
The activity for peak A (1653) and peak B(2310.9) but the third peak C was very low.
while maximum specific activity was obtained in the second peak (B) which showed (23107), (18366 ) IU/ml /mg for (A) and very low for the third one (C), and (12.58),(26.531) folds of purification for B and A peaks respectively .Furthermore, the comparative molecular weight of the partially purified isoenzymes arylesterase peaks B and A) using gel filtration were found to be (92,129), (86,895) Dalton respectively.
The optimum conditions of arylesterase were determined as maximum activity was obtained using (9 mM) Tris – HCl as a buffer at pH (7.2), with incubation temperature (37ºC), incubation time (25min) and (4mM) of phenyl acetate as a substrate.
Using Linweaver–Burk plot, it was found that maximum velocity (Vmax) and Michaela’s constant (Km) had the values of (211UI/ml) and (2.8 mM) respectively.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Almashhdani, Firdaws A.& Bakr, Lama Abd al-Munim& Abd Allah, Sayrawan Ahmad. 2017. Extraction and characterization of Arylesterase enzyme from (peel and pulp) of Annona muricata fruit. Kirkuk University Journal-Scientific Studies،Vol. 12, no. 2.
https://search.emarefa.net/detail/BIM-946288
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Almashhdani, Firdaws A.…[et al.]. Extraction and characterization of Arylesterase enzyme from (peel and pulp) of Annona muricata fruit. Kirkuk University Journal-Scientific Studies Vol. 12, no. 2 (Mar. 2017).
https://search.emarefa.net/detail/BIM-946288
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Almashhdani, Firdaws A.& Bakr, Lama Abd al-Munim& Abd Allah, Sayrawan Ahmad. Extraction and characterization of Arylesterase enzyme from (peel and pulp) of Annona muricata fruit. Kirkuk University Journal-Scientific Studies. 2017. Vol. 12, no. 2.
https://search.emarefa.net/detail/BIM-946288
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
رقم السجل
BIM-946288
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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