Extraction and characterization of Arylesterase enzyme from (peel and pulp) of Annona muricata fruit
Other Title(s)
استخلاص و توصيف انزيم اريل استيريز من لب و قشره ثمره القشطه
Joint Authors
Almashhdani, Firdaws A.
Bakr, Lama Abd al-Munim
Abd Allah, Sayrawan Ahmad
Source
Kirkuk University Journal-Scientific Studies
Issue
Vol. 12, Issue 2 (31 Mar. 2017)24 p.
Publisher
Kirkuk University College of Science
Publication Date
2017-03-31
Country of Publication
Iraq
No. of Pages
24
Main Subjects
Abstract EN
The extraction of arylesterase from the aqueous extract of (peel and pulp) of Annona muricata fruit was conducted using different biochemical techniques.
It was shown that, max activity was obtained in the pulp than in peel, and by using gel filtration chromatography on sephadex G-75 for the pulp part, the solution of the proteinous precipitate produced by acetone precipitation, contained three proteinous peaks.
The activity for peak A (1653) and peak B(2310.9) but the third peak C was very low.
while maximum specific activity was obtained in the second peak (B) which showed (23107), (18366 ) IU/ml /mg for (A) and very low for the third one (C), and (12.58),(26.531) folds of purification for B and A peaks respectively .Furthermore, the comparative molecular weight of the partially purified isoenzymes arylesterase peaks B and A) using gel filtration were found to be (92,129), (86,895) Dalton respectively.
The optimum conditions of arylesterase were determined as maximum activity was obtained using (9 mM) Tris – HCl as a buffer at pH (7.2), with incubation temperature (37ºC), incubation time (25min) and (4mM) of phenyl acetate as a substrate.
Using Linweaver–Burk plot, it was found that maximum velocity (Vmax) and Michaela’s constant (Km) had the values of (211UI/ml) and (2.8 mM) respectively.
American Psychological Association (APA)
Almashhdani, Firdaws A.& Bakr, Lama Abd al-Munim& Abd Allah, Sayrawan Ahmad. 2017. Extraction and characterization of Arylesterase enzyme from (peel and pulp) of Annona muricata fruit. Kirkuk University Journal-Scientific Studies،Vol. 12, no. 2.
https://search.emarefa.net/detail/BIM-946288
Modern Language Association (MLA)
Almashhdani, Firdaws A.…[et al.]. Extraction and characterization of Arylesterase enzyme from (peel and pulp) of Annona muricata fruit. Kirkuk University Journal-Scientific Studies Vol. 12, no. 2 (Mar. 2017).
https://search.emarefa.net/detail/BIM-946288
American Medical Association (AMA)
Almashhdani, Firdaws A.& Bakr, Lama Abd al-Munim& Abd Allah, Sayrawan Ahmad. Extraction and characterization of Arylesterase enzyme from (peel and pulp) of Annona muricata fruit. Kirkuk University Journal-Scientific Studies. 2017. Vol. 12, no. 2.
https://search.emarefa.net/detail/BIM-946288
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-946288