Association study of HSD11B1 rs12086634 (T>G)‎ gene polymorphism with polycystic ovarian syndrome in Erbil Province

الملخص EN

Background: Polycystic ovarian syndrome (PCOS) is one of the most prevalent gynaecological imbalances of endocrine hormones and metabolic disorders that affect women during puberty, with a prevalence of up to 17.8%.

Objective: The current study focused on the presence of rs12086634 T>G genetic variant of 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1) gene polymorphism in Erbil province PCOS women.

Participants and methods: The present study was conducted on 104 PCOS women and 94 control women to investigate the association HSD11B1 rs12086634 (T>G) gene polymorphism with PCOS.

The extracted genomic DNA from whole blood according to the protocol provided by the manufacturer company.

HSD11B1 rs12086634 T>G gene polymorphism was detected by tetra primer-amplification refractory mutation system based polymerase chain reaction (T-ARMS-PCR) method.

Result: The obtained results suggest that the frequency of the HSD11B1 TG and GG genotypes in women with PCOS increased 3.60-fold and 4.39-fold compared to the control group (odds ratio [OR]; 3.60 and 4.39, 95% confidence interval [CI], 1.951-6.668 and 0.175-110.2; P-value <0.0001 and 0.22) respectively, the body mass index of women with G-allele was significantly greater than women with T-Allele (28.32 kg/ mt2 vs.

26.20 kg/ mt2,and p = 0.0256).

Conclusions: Our findings indicated and proved that the findings indicate that both homozygous and heterozygous genotypes of HSD11B1 rs12086634 T>G gene polymorphism was associated with PCOS in Erbil province.