Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.)
العناوين الأخرى
زراعة الأجنة و الإكثار الدقيق خارج الجسم الحي للبلوط (Quercus aegilops L.)
المؤلفون المشاركون
Fadl al-Din, Laylan Husayn
Tuma, Rafail Shlimon
المصدر
The Iraqi Journal of Agricultural Science
العدد
المجلد 51، العدد 1 (29 فبراير/شباط 2020)، ص ص. 347-355، 9ص.
الناشر
تاريخ النشر
2020-02-29
دولة النشر
العراق
عدد الصفحات
9
التخصصات الرئيسية
الموضوعات
الملخص EN
An attempt was done to develop a micropropagation protocol for oak using embryo culture.
Oak is considered a hard-to-root woody plant by conventional propagation methods, that’s why using tissue culture techniques is a very suitable alternative method.
For oak embryo culture, WPM was used and found to be better than MS medium for embryo germination which gave 66.13%.
As well as adding of GA3 to the medium improved the germination rate of embryos (43.25% and 82.25 %).
At initiation stage, WPM was used and found to be the best medium by giving the highest number of shoots/ explant which was 1.80, the highest number of leaves (15.17 leaves/ explant) and the longest shoots (1.42 cm) followed by MS medium then GD which gave the lowest parameters which gave 0.98 shoots/ explant, 7.20 leaves/ explant and 1.06 cm shoot length.
At shoot multiplication stage, BA was better than Kinetin for multiplication of oak explants.
The addition of BA at 3 mg.l-l gave the highest number of shoot and leaves which were 3.33 and 26.11 respectively.
The longest shoots were achieved when 4.5 mg.l-l of BA was used.
Furthermore, kinetin at 3 and 4.5 mg-l gave the lowest parameters which were 1 cm in length and 1.54 leaves/ explant.
For rooting stage, NAA was better than IAA in giving better parameters and rooting percentage.
The highest number of roots and rooting percentage were achieved when 1 mg.l-l was added by giving 6 roots/ explant and 100% rooting percentage.
While the longest roots were achieved when 0.5 mg.l-l of NAA was used (3.67 cm) followed by 1.5 mg.l-l IAA which gave 3.55 roots/ explant with rooting percentage 90%.
The produced plantlets were successfully acclimatized and transferred to the open-air conditions with a rate reached 85%.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fadl al-Din, Laylan Husayn& Tuma, Rafail Shlimon. 2020. Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.). The Iraqi Journal of Agricultural Science،Vol. 51, no. 1, pp.347-355.
https://search.emarefa.net/detail/BIM-948710
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fadl al-Din, Laylan Husayn& Tuma, Rafail Shlimon. Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.). The Iraqi Journal of Agricultural Science Vol. 51, no. 1 (2020), pp.347-355.
https://search.emarefa.net/detail/BIM-948710
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fadl al-Din, Laylan Husayn& Tuma, Rafail Shlimon. Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.). The Iraqi Journal of Agricultural Science. 2020. Vol. 51, no. 1, pp.347-355.
https://search.emarefa.net/detail/BIM-948710
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 354-355
رقم السجل
BIM-948710
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر