Study the protective effect of radish (Raphanus sativus)‎ seeds extract against harmful effects of sodium nitrite on some physiological and histological parameters in male rabbits

الملخص EN

The aim of this study is to investigate the protective effect of Radish (Raphanus sativus) seeds ethanolic extract 70% against harmful effects of sodium nitrite (NaNOR2R)R Ron some physiological and histological parameters of liver and kidney in male rabbits.R R Twenty five adult male rabbits were used in this study, divided into five groups (five rabbits in each group) and intubated daily for 30 days.

Group T1: intubated orally 20 mg/kg NaNOR2R, Group T2: intubated orally 20 mg/kg NaNOR2R + 50 mg/kg of ethanolic extract from Raphanus sativus seeds, Group T3: intubated orally 20 mg/kg NaNOR2R + 100 mg/kg of ethanolic extract from Raphanus sativus seeds, Group T4: intubated orally 20 mg/kg NaNOR2R + 200 mg/kg of ethanolic extract from Raphanus sativus seeds as well as Group C: control intubated orally distilled water.

In rabbits intubated with NaNOR2R, the results showed that there were increasing in the level of liver enzymes (AST, ALT and ALP) and kidney functions parameters (Uric acid and Creatinine) in comparison with normal rabbits (control).

In rabbits intubated with NaNOR2R + 50,100,200 mg/kg of ethanolic extract of Raphanus Sativus seeds, this study explained that there were decreasing in the level of liver enzymes (AST, ALT and ALP) and kidney functions parameters (uric acid and creatinine) in comparison with rabbits intubated with NaNOR2 Ronly.

Histological sections of liver and kidney of sodium nitrite treated group revealed congestion of blood vessels, infiltration, fatty degeneration, While histological examination findings of sodium nitrite + Raphanus sativus seeds extract treated groups revealed healing of liver and kidney tissue.

In conclusion according to results obtained from this study that alcoholic extract of Raphanus sativus seeds in dose (50,100,200 mg/kg) have protective role against harmful effect of sodium nitrite on liver and kidney in male rabbits