Antibiotic resistant patterns of bacteria recovered from infected diabetic foot as correlated to risk factors

العناوين الأخرى

أنماط مقاومة الجراثيم المعزولة من خمج قدم مرضى السكري و علاقتها ببعض عوامل الخطورة

مقدم أطروحة جامعية

Ali, Muhammad Shakir

مشرف أطروحة جامعية

al-Hadithi, Hadil Tawfiq

الجامعة

جامعة الإسراء

الكلية

كلية الصيدلة

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2020

الملخص الإنجليزي

Background: Incidence of diabetes is rising worldwide, and the prevalence of diabetes for all age-groups worldwide was estimated to be 2.8% in 2000 and 4.4% in 2030.

Contaminating bacteria among diabetic foot can couse foot ulceration and this can develop to gangarin then ampotation.

Aim of the study: Collecte Samples from diabetic foot infection (DFI) lesions at National Centre for diabetes endocrinology and genetics (NCDEG) diabetic foot clinic / University of Jordan, to isolate and identify contaminating bacteria, correlate their frequencies with risk factors, and study their antibiotics resistance patterns.

Patient and Methods: Seventy diabetic outpatients (Type II) aged above 30 years, with diabetic foot infection (DFI) attending NCDEG during the period from October 2019- Mid March 2020 were included in the study.

A structural questionnaire (Appendix A) was designed to collect data through interviewing patients in addition to medical records.

Swabs from DFI lesions were cultured onto a number of bacteriological media.

methicillin resistant Staphelococcus aureus (MRSA) and Methicillin-resistant staphylococcus aureus (MRCONS) were detected by growing isolates on Mannitol salt agar (MSA) and Mueller-Hinton agar supplemented with 6ug/ml Oxacillin.

An assortment of antibiotics against Gram negatev and Gram positive were examined to explore antibiotic resistance patterns.

Results:DFI are more prevalent in elderly patients aged > 50years (88.6%), male are more affected than females.

HbAIC was uncontrolled in 70.0% of patients and 47.1% of them have a duration of diabetes for more than 30 years.

Number of patients not committed to diet is 60% and 74.3% are non-smokers.

A significant correlation was found between HbAlC values with gender and diet (p<0.05).

A Similar incidence of DFI was reported by patients on either oral or insulin medication (17: 24.3% each).

36 patients (51.4%) were on both oral and insulin medication.

The duration of foot complication (28.6 %) of patients has lasted for more than 6 months.

Gram positive was the predominating bacteria isolated from DFI lesions.

S.aureus was the largest group (51.4%) followed by CONs (47.1%) and Enterococcus (12.9%).

MRSA and MRCONs were recovered from 40 % and 30% of study participants respectively.

The highest percentage recovery of MRSA and MRCONs were recorded among patients with duration of foot infection of 6 months - 1year (75% and 50% respectively).

Percentage frequencies of major Gram negative bacteria as following: Proteus mirabilis 18.6%, Pseudomonas aeruginosa 14.3%, and Acinetobacter spp.7.1%.

High frequencies of S.

aureus (55.6%), P.

aeruginosa (50%) and Kleb.

penumoniae (40%) were recovered among patients having surgical amputation.

Conculution: MDROs were prevalent amongst Gm+ve and Gm-ve isolates at alarming levels.

S.

aureus and Groub B streptococci (GBS) were resistant to seven and six antibiotics respectively.

Vancomycin, Linezolid, Teicoplanin and Tigecycline were the most effective against Gram positive organisms.

Gm-ve bacteria showed resistance to varied numbers (8-11)of antibiotics Only Prot.

mirabilis was resistant to Tigecycline (100%).

التخصصات الرئيسية

علم الصيدلة

الموضوعات

• عوامل الخطر
• قرحة القدم السكرية
• مقاومة المضادات الحيوية

عدد الصفحات

106

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-985109