Competition of Lactobacillus paracasei with Salmonella enterica for Adhesion to Caco-2 Cells
المؤلفون المشاركون
Laubitz, Daniel
Zabielski, Romuald
Antushevich, Hanna
Grzesiuk, Elżbieta
Jankowska, Alicja
المصدر
العدد
المجلد 2008، العدد 2008 (31 ديسمبر/كانون الأول 2008)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2008-02-24
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Competition of commensal and probiotic bacteria with pathogens for adhesion and colonization is one of the important protective mechanisms of gastrointestinal tract.
In this study, we examined the ability of Lactobacillus paracasei to inhibit the adhesion of pathogenic Salmonella enterica to human colon adenocarcinoma Caco-2 cells.
Caco-2 cells were grown for 6 or 21 days to obtain nondifferentiated or well-differentiated cells, respectively.
In adhesion experiments, bacteria were added to the cells for 2 or 4 hours.
The number of attached bacteria was expressed as colony-forming units (CFUs), Caco-2 cells were counted in hematocytometer.
Both bacterial strains used adhered better to well-differentiated than to nondifferentiated Caco-2 cells, however, the amount of Salmonella adhered to Caco-2 after 2 hours of contact was 12-fold higher in comparison to L.
paracasei and almost 27-fold higher after 4 hours of contact.
Two types of experiments were done: coincubation (both bacteria were added to Caco-2 cells simultaneously), and preincubation (L.
paracasei was incubated with Caco-2 cells first, and then S.
enterica was added).
In coincubation experiment, the presence of L.
paracasei decreased S.
enterica adhesion by 4-fold and in preincubation experiment even 7-fold.
Generally, Lactobacillus spent culture supernatants (SCSs) acted weaker as inhibitors of Salmonella adhesion in comparison to the whole L.
paracasei culture in coincubation experiment.
In conclusion, the displacement of pathogens by lactic acid bacteria and its secretions showed here depends on the time of bacteria-epithelial cell contact, and also on the stage of Caco-2 differentiation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jankowska, Alicja& Laubitz, Daniel& Antushevich, Hanna& Zabielski, Romuald& Grzesiuk, Elżbieta. 2008. Competition of Lactobacillus paracasei with Salmonella enterica for Adhesion to Caco-2 Cells. BioMed Research International،Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-987815
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jankowska, Alicja…[et al.]. Competition of Lactobacillus paracasei with Salmonella enterica for Adhesion to Caco-2 Cells. BioMed Research International No. 2008 (2008), pp.1-6.
https://search.emarefa.net/detail/BIM-987815
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jankowska, Alicja& Laubitz, Daniel& Antushevich, Hanna& Zabielski, Romuald& Grzesiuk, Elżbieta. Competition of Lactobacillus paracasei with Salmonella enterica for Adhesion to Caco-2 Cells. BioMed Research International. 2008. Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-987815
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-987815
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر