Competition of Lactobacillus paracasei with Salmonella enterica for Adhesion to Caco-2 Cells
Joint Authors
Laubitz, Daniel
Zabielski, Romuald
Antushevich, Hanna
Grzesiuk, Elżbieta
Jankowska, Alicja
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-02-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Competition of commensal and probiotic bacteria with pathogens for adhesion and colonization is one of the important protective mechanisms of gastrointestinal tract.
In this study, we examined the ability of Lactobacillus paracasei to inhibit the adhesion of pathogenic Salmonella enterica to human colon adenocarcinoma Caco-2 cells.
Caco-2 cells were grown for 6 or 21 days to obtain nondifferentiated or well-differentiated cells, respectively.
In adhesion experiments, bacteria were added to the cells for 2 or 4 hours.
The number of attached bacteria was expressed as colony-forming units (CFUs), Caco-2 cells were counted in hematocytometer.
Both bacterial strains used adhered better to well-differentiated than to nondifferentiated Caco-2 cells, however, the amount of Salmonella adhered to Caco-2 after 2 hours of contact was 12-fold higher in comparison to L.
paracasei and almost 27-fold higher after 4 hours of contact.
Two types of experiments were done: coincubation (both bacteria were added to Caco-2 cells simultaneously), and preincubation (L.
paracasei was incubated with Caco-2 cells first, and then S.
enterica was added).
In coincubation experiment, the presence of L.
paracasei decreased S.
enterica adhesion by 4-fold and in preincubation experiment even 7-fold.
Generally, Lactobacillus spent culture supernatants (SCSs) acted weaker as inhibitors of Salmonella adhesion in comparison to the whole L.
paracasei culture in coincubation experiment.
In conclusion, the displacement of pathogens by lactic acid bacteria and its secretions showed here depends on the time of bacteria-epithelial cell contact, and also on the stage of Caco-2 differentiation.
American Psychological Association (APA)
Jankowska, Alicja& Laubitz, Daniel& Antushevich, Hanna& Zabielski, Romuald& Grzesiuk, Elżbieta. 2008. Competition of Lactobacillus paracasei with Salmonella enterica for Adhesion to Caco-2 Cells. BioMed Research International،Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-987815
Modern Language Association (MLA)
Jankowska, Alicja…[et al.]. Competition of Lactobacillus paracasei with Salmonella enterica for Adhesion to Caco-2 Cells. BioMed Research International No. 2008 (2008), pp.1-6.
https://search.emarefa.net/detail/BIM-987815
American Medical Association (AMA)
Jankowska, Alicja& Laubitz, Daniel& Antushevich, Hanna& Zabielski, Romuald& Grzesiuk, Elżbieta. Competition of Lactobacillus paracasei with Salmonella enterica for Adhesion to Caco-2 Cells. BioMed Research International. 2008. Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-987815
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-987815