C-Peptide and Its C-Terminal Fragments Improve Erythrocyte Deformability in Type 1 Diabetes Patients
المؤلفون المشاركون
Pfützner, Andreas
Wahren, John
Forst, Thomas
Ekberg, Karin
Hach, Thomas
Kunt, Thomas
المصدر
العدد
المجلد 2008، العدد 2008 (31 ديسمبر/كانون الأول 2008)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2008-04-01
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Aims/hypothesis.
Data now indicate that proinsulin C-peptide exerts important physiological effects and shows the characteristics of an endogenous peptide hormone.
This study aimed to investigate the influence of C-peptide and fragments thereof on erythrocyte deformability and to elucidate the relevant signal transduction pathway.
Methods.
Blood samples from 23 patients with type 1 diabetes and 15 matched healthy controls were incubated with 6.6 nM of either human C-peptide, C-terminal hexapeptide, C-terminal pentapeptide, a middle fragment comprising residues 11–19 of C-peptide, or randomly scrambled C-peptide.
Furthermore, red blood cells from 7 patients were incubated with C-peptide, penta- and hexapeptides with/without addition of ouabain, EDTA, or pertussis toxin.
Erythrocyte deformability was measured using a laser diffractoscope in the shear stress range 0.3–60 Pa.
Results.
Erythrocyte deformability was impaired by 18–25% in type 1 diabetic patients compared to matched controls in the physiological shear stress range 0.6–12 Pa (P<.01–.001).
C-peptide, penta- and hexapeptide all significantly improved the impaired erythrocyte deformability of type 1 diabetic patients, while the middle fragment and scrambled C-peptide had no detectable effect.
Treatment of erythrocytes with ouabain or EDTA completely abolished the C-peptide, penta- and hexapeptide effects.
Pertussis toxin in itself significantly increased erythrocyte deformability.
Conclusion/interpretation.
C-peptide and its C-terminal fragments are equally effective in improving erythrocyte deformability in type 1 diabetes.
The C-terminal residues of C-peptide are causally involved in this effect.
The signal transduction pathway is Ca2+-dependent and involves activation of red blood cell Na+,K+-ATPase.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hach, Thomas& Forst, Thomas& Kunt, Thomas& Ekberg, Karin& Pfützner, Andreas& Wahren, John. 2008. C-Peptide and Its C-Terminal Fragments Improve Erythrocyte Deformability in Type 1 Diabetes Patients. Journal of Diabetes Research،Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-988020
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hach, Thomas…[et al.]. C-Peptide and Its C-Terminal Fragments Improve Erythrocyte Deformability in Type 1 Diabetes Patients. Journal of Diabetes Research No. 2008 (2008), pp.1-6.
https://search.emarefa.net/detail/BIM-988020
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hach, Thomas& Forst, Thomas& Kunt, Thomas& Ekberg, Karin& Pfützner, Andreas& Wahren, John. C-Peptide and Its C-Terminal Fragments Improve Erythrocyte Deformability in Type 1 Diabetes Patients. Journal of Diabetes Research. 2008. Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-988020
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-988020
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر