C-Peptide and Its C-Terminal Fragments Improve Erythrocyte Deformability in Type 1 Diabetes Patients
Joint Authors
Pfützner, Andreas
Wahren, John
Forst, Thomas
Ekberg, Karin
Hach, Thomas
Kunt, Thomas
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-04-01
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aims/hypothesis.
Data now indicate that proinsulin C-peptide exerts important physiological effects and shows the characteristics of an endogenous peptide hormone.
This study aimed to investigate the influence of C-peptide and fragments thereof on erythrocyte deformability and to elucidate the relevant signal transduction pathway.
Methods.
Blood samples from 23 patients with type 1 diabetes and 15 matched healthy controls were incubated with 6.6 nM of either human C-peptide, C-terminal hexapeptide, C-terminal pentapeptide, a middle fragment comprising residues 11–19 of C-peptide, or randomly scrambled C-peptide.
Furthermore, red blood cells from 7 patients were incubated with C-peptide, penta- and hexapeptides with/without addition of ouabain, EDTA, or pertussis toxin.
Erythrocyte deformability was measured using a laser diffractoscope in the shear stress range 0.3–60 Pa.
Results.
Erythrocyte deformability was impaired by 18–25% in type 1 diabetic patients compared to matched controls in the physiological shear stress range 0.6–12 Pa (P<.01–.001).
C-peptide, penta- and hexapeptide all significantly improved the impaired erythrocyte deformability of type 1 diabetic patients, while the middle fragment and scrambled C-peptide had no detectable effect.
Treatment of erythrocytes with ouabain or EDTA completely abolished the C-peptide, penta- and hexapeptide effects.
Pertussis toxin in itself significantly increased erythrocyte deformability.
Conclusion/interpretation.
C-peptide and its C-terminal fragments are equally effective in improving erythrocyte deformability in type 1 diabetes.
The C-terminal residues of C-peptide are causally involved in this effect.
The signal transduction pathway is Ca2+-dependent and involves activation of red blood cell Na+,K+-ATPase.
American Psychological Association (APA)
Hach, Thomas& Forst, Thomas& Kunt, Thomas& Ekberg, Karin& Pfützner, Andreas& Wahren, John. 2008. C-Peptide and Its C-Terminal Fragments Improve Erythrocyte Deformability in Type 1 Diabetes Patients. Journal of Diabetes Research،Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-988020
Modern Language Association (MLA)
Hach, Thomas…[et al.]. C-Peptide and Its C-Terminal Fragments Improve Erythrocyte Deformability in Type 1 Diabetes Patients. Journal of Diabetes Research No. 2008 (2008), pp.1-6.
https://search.emarefa.net/detail/BIM-988020
American Medical Association (AMA)
Hach, Thomas& Forst, Thomas& Kunt, Thomas& Ekberg, Karin& Pfützner, Andreas& Wahren, John. C-Peptide and Its C-Terminal Fragments Improve Erythrocyte Deformability in Type 1 Diabetes Patients. Journal of Diabetes Research. 2008. Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-988020
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988020