Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients

المؤلفون المشاركون

Mele, Raffaella
Ravà, Lucilla
Giuliani, Laura
Crinò, Antonino
Altieri, Laura
Di Giovine, Monica
Fierabracci, Alessandra

المصدر

BioMed Research International

العدد

المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2009-12-02

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الطب البشري

الملخص EN

Type 1 diabetes (T1D) is an autoimmune disease, in which pancreatic β cells are destroyed in genetically predisposed individuals.

While the direct contribution of autoantibodies to the disease pathogenesis is controversial, it is generally recognised that the mechanism of β cell destruction is mediated by autoreactive T cells that had escaped the thymic selection.

We aimed to design a method to detect circulating CD8+ T cells autoreactive against an epitope of the glutamic acid decarboxylase autoantigen, isoform 65 (GAD65) ex vivo in T1D patients by using HLA class I tetramers.

Low frequencies of GAD65 peptide-specific CD8+ cytotoxic T lymphocytes were detected in peripheral blood lymphocytes (PBMC) of normal controls after GAD65 peptide-specific stimulation.

Conversely, their frequencies were significantly higher than in controls in PBMC of T1D patients after GAD65 peptide stimulation.

These preliminary data are encouraging in order to develop a reliable assay to be employed in large-scale screening studies.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Giuliani, Laura& Mele, Raffaella& Di Giovine, Monica& Altieri, Laura& Crinò, Antonino& Ravà, Lucilla…[et al.]. 2009. Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients. BioMed Research International،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988396

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Giuliani, Laura…[et al.]. Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients. BioMed Research International No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-988396

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Giuliani, Laura& Mele, Raffaella& Di Giovine, Monica& Altieri, Laura& Crinò, Antonino& Ravà, Lucilla…[et al.]. Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988396

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-988396