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Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients
Joint Authors
Mele, Raffaella
Ravà, Lucilla
Giuliani, Laura
Crinò, Antonino
Altieri, Laura
Di Giovine, Monica
Fierabracci, Alessandra
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Type 1 diabetes (T1D) is an autoimmune disease, in which pancreatic β cells are destroyed in genetically predisposed individuals.
While the direct contribution of autoantibodies to the disease pathogenesis is controversial, it is generally recognised that the mechanism of β cell destruction is mediated by autoreactive T cells that had escaped the thymic selection.
We aimed to design a method to detect circulating CD8+ T cells autoreactive against an epitope of the glutamic acid decarboxylase autoantigen, isoform 65 (GAD65) ex vivo in T1D patients by using HLA class I tetramers.
Low frequencies of GAD65 peptide-specific CD8+ cytotoxic T lymphocytes were detected in peripheral blood lymphocytes (PBMC) of normal controls after GAD65 peptide-specific stimulation.
Conversely, their frequencies were significantly higher than in controls in PBMC of T1D patients after GAD65 peptide stimulation.
These preliminary data are encouraging in order to develop a reliable assay to be employed in large-scale screening studies.
American Psychological Association (APA)
Giuliani, Laura& Mele, Raffaella& Di Giovine, Monica& Altieri, Laura& Crinò, Antonino& Ravà, Lucilla…[et al.]. 2009. Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients. BioMed Research International،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988396
Modern Language Association (MLA)
Giuliani, Laura…[et al.]. Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients. BioMed Research International No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-988396
American Medical Association (AMA)
Giuliani, Laura& Mele, Raffaella& Di Giovine, Monica& Altieri, Laura& Crinò, Antonino& Ravà, Lucilla…[et al.]. Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988396
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988396