Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer

المؤلفون المشاركون

Liu, Ke
Wang, Guo-Bin
Lu, Xiao-Ming
Shu, Xiao-Gang
Tong, Qiang

المصدر

BioMed Research International

العدد

المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2010-03-08

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الطب البشري

الملخص EN

Antibody-targeted superantigen has been developed into a new strategy to treat many malignant tumors.

In this study, for specific targeting to gastric cancer cell, superantigen SEB (Staphylococcal Enterotoxin B) was genetically fused to the single-chain variable fragment of gastric carcinoma-associated antibody MG7(MG7-scFv) that recognizes the MG7 antigen frequently expressed in gastric cancer cell.

The recombinant MG7-scFv/SEB fusion proteins are expressed in E.

coli as inclusion bodies, and the purified MG7-scFv/SEB retains high binding affinity with gastric cancer cell SGC-7901 (positive MG7 antigen expression).

When incubated with effector cell—peripheral blood mononuclear cells (PBMCs), MG7-scFv/SEB could effectively inhibit the proliferation and induce apoptosis of SGC-7901.

After being treated with MG7-scFv/SEB, PBMCs remarkably increased the production of Th1 cytokines (IFN-gamma, IL-2), and slightly increased the production of Th2 cytokines (IL-4, IL-10) in vitro.

It was observed that gastric-tumor-bearing rats administrated with MG7-scFv/SEB showed more inflammatory cell infiltration, more significant tumor inhibition, and longer survival time than those of rats treated with SEB or NS (Normal Saline).

The data indicated that MG7-scFv/SEB fusion protein could specifically target gastric cancer cell, enhance the activity of T cells and induce tumor cell apoptosis to exert the antitumor effect on gastric cancer.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Tong, Qiang& Liu, Ke& Lu, Xiao-Ming& Shu, Xiao-Gang& Wang, Guo-Bin. 2010. Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer. BioMed Research International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988760

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Tong, Qiang…[et al.]. Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer. BioMed Research International No. 2010 (Dec. 2010), pp.1-8.
https://search.emarefa.net/detail/BIM-988760

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Tong, Qiang& Liu, Ke& Lu, Xiao-Ming& Shu, Xiao-Gang& Wang, Guo-Bin. Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988760

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-988760