Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer
المؤلفون المشاركون
Liu, Ke
Wang, Guo-Bin
Lu, Xiao-Ming
Shu, Xiao-Gang
Tong, Qiang
المصدر
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-03-08
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Antibody-targeted superantigen has been developed into a new strategy to treat many malignant tumors.
In this study, for specific targeting to gastric cancer cell, superantigen SEB (Staphylococcal Enterotoxin B) was genetically fused to the single-chain variable fragment of gastric carcinoma-associated antibody MG7(MG7-scFv) that recognizes the MG7 antigen frequently expressed in gastric cancer cell.
The recombinant MG7-scFv/SEB fusion proteins are expressed in E.
coli as inclusion bodies, and the purified MG7-scFv/SEB retains high binding affinity with gastric cancer cell SGC-7901 (positive MG7 antigen expression).
When incubated with effector cell—peripheral blood mononuclear cells (PBMCs), MG7-scFv/SEB could effectively inhibit the proliferation and induce apoptosis of SGC-7901.
After being treated with MG7-scFv/SEB, PBMCs remarkably increased the production of Th1 cytokines (IFN-gamma, IL-2), and slightly increased the production of Th2 cytokines (IL-4, IL-10) in vitro.
It was observed that gastric-tumor-bearing rats administrated with MG7-scFv/SEB showed more inflammatory cell infiltration, more significant tumor inhibition, and longer survival time than those of rats treated with SEB or NS (Normal Saline).
The data indicated that MG7-scFv/SEB fusion protein could specifically target gastric cancer cell, enhance the activity of T cells and induce tumor cell apoptosis to exert the antitumor effect on gastric cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tong, Qiang& Liu, Ke& Lu, Xiao-Ming& Shu, Xiao-Gang& Wang, Guo-Bin. 2010. Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer. BioMed Research International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988760
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tong, Qiang…[et al.]. Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer. BioMed Research International No. 2010 (Dec. 2010), pp.1-8.
https://search.emarefa.net/detail/BIM-988760
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tong, Qiang& Liu, Ke& Lu, Xiao-Ming& Shu, Xiao-Gang& Wang, Guo-Bin. Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988760
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-988760
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر