Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer
Joint Authors
Liu, Ke
Wang, Guo-Bin
Lu, Xiao-Ming
Shu, Xiao-Gang
Tong, Qiang
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Antibody-targeted superantigen has been developed into a new strategy to treat many malignant tumors.
In this study, for specific targeting to gastric cancer cell, superantigen SEB (Staphylococcal Enterotoxin B) was genetically fused to the single-chain variable fragment of gastric carcinoma-associated antibody MG7(MG7-scFv) that recognizes the MG7 antigen frequently expressed in gastric cancer cell.
The recombinant MG7-scFv/SEB fusion proteins are expressed in E.
coli as inclusion bodies, and the purified MG7-scFv/SEB retains high binding affinity with gastric cancer cell SGC-7901 (positive MG7 antigen expression).
When incubated with effector cell—peripheral blood mononuclear cells (PBMCs), MG7-scFv/SEB could effectively inhibit the proliferation and induce apoptosis of SGC-7901.
After being treated with MG7-scFv/SEB, PBMCs remarkably increased the production of Th1 cytokines (IFN-gamma, IL-2), and slightly increased the production of Th2 cytokines (IL-4, IL-10) in vitro.
It was observed that gastric-tumor-bearing rats administrated with MG7-scFv/SEB showed more inflammatory cell infiltration, more significant tumor inhibition, and longer survival time than those of rats treated with SEB or NS (Normal Saline).
The data indicated that MG7-scFv/SEB fusion protein could specifically target gastric cancer cell, enhance the activity of T cells and induce tumor cell apoptosis to exert the antitumor effect on gastric cancer.
American Psychological Association (APA)
Tong, Qiang& Liu, Ke& Lu, Xiao-Ming& Shu, Xiao-Gang& Wang, Guo-Bin. 2010. Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer. BioMed Research International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988760
Modern Language Association (MLA)
Tong, Qiang…[et al.]. Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer. BioMed Research International No. 2010 (Dec. 2010), pp.1-8.
https://search.emarefa.net/detail/BIM-988760
American Medical Association (AMA)
Tong, Qiang& Liu, Ke& Lu, Xiao-Ming& Shu, Xiao-Gang& Wang, Guo-Bin. Construction and Characterization of a Novel Fusion Protein MG7-scFvSEB against Gastric Cancer. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988760
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988760