Quantitative Proteomics Analysis of Maternal Plasma in Down Syndrome Pregnancies Using Isobaric Tagging Reagent (iTRAQ)
المؤلفون المشاركون
Hahn, Sinuhe
Kolla, Varaprasad
Moes, Suzette
Tercanli, Sevgi
Lapaire, Olav
Choolani, Mahesh
Jenö, Paul
المصدر
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-11-05
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Currently no specific biomarkers exist for the screening of pregnancies at risk for down syndrome (DS).
Since a quantitative proteomic approach with isobaric labelling (iTRAQ) has recently been suggested to be highly suitable for the discovery of novel plasma biomarkers, we have now used this method to examine for potential quantitative changes in the plasma proteome of the pregnancies bearing DS fetuses in comparison to normal healthy babies.
In our study, we used plasma from six women with DS pregnancies and six with uncomplicated pregnancies care were taken to match cases and controls for gestational and maternal age, as these could be a confounder.
In our quantitative proteomics analysis we were able to detect 178 proteins using iTRAQ labelling in conjunction with 4800 MALDI TOF/TOF.
Amongst these we observed changes in βHCG, a known screening marker for DS, indicating that our assay was functional.
We found a number of elevated proteins Ig lambda chain C region, serum amyloid P-component, amyloid beta A4, and under expressed proteins like gamma-actin and titin in DS pregnancies.
These proteins are also found in the sera of patients with Alzheimer disease, which share similar pathologies of DS.
Our study therefore indicates that the iTRAQ labelling approach may be indeed useful for the detection of novel biomarkers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kolla, Varaprasad& Jenö, Paul& Moes, Suzette& Tercanli, Sevgi& Lapaire, Olav& Choolani, Mahesh…[et al.]. 2009. Quantitative Proteomics Analysis of Maternal Plasma in Down Syndrome Pregnancies Using Isobaric Tagging Reagent (iTRAQ). BioMed Research International،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-989203
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kolla, Varaprasad…[et al.]. Quantitative Proteomics Analysis of Maternal Plasma in Down Syndrome Pregnancies Using Isobaric Tagging Reagent (iTRAQ). BioMed Research International No. 2010 (Dec. 2010), pp.1-10.
https://search.emarefa.net/detail/BIM-989203
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kolla, Varaprasad& Jenö, Paul& Moes, Suzette& Tercanli, Sevgi& Lapaire, Olav& Choolani, Mahesh…[et al.]. Quantitative Proteomics Analysis of Maternal Plasma in Down Syndrome Pregnancies Using Isobaric Tagging Reagent (iTRAQ). BioMed Research International. 2009. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-989203
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-989203
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر