Quantitative Proteomics Analysis of Maternal Plasma in Down Syndrome Pregnancies Using Isobaric Tagging Reagent (iTRAQ)
Joint Authors
Hahn, Sinuhe
Kolla, Varaprasad
Moes, Suzette
Tercanli, Sevgi
Lapaire, Olav
Choolani, Mahesh
Jenö, Paul
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Currently no specific biomarkers exist for the screening of pregnancies at risk for down syndrome (DS).
Since a quantitative proteomic approach with isobaric labelling (iTRAQ) has recently been suggested to be highly suitable for the discovery of novel plasma biomarkers, we have now used this method to examine for potential quantitative changes in the plasma proteome of the pregnancies bearing DS fetuses in comparison to normal healthy babies.
In our study, we used plasma from six women with DS pregnancies and six with uncomplicated pregnancies care were taken to match cases and controls for gestational and maternal age, as these could be a confounder.
In our quantitative proteomics analysis we were able to detect 178 proteins using iTRAQ labelling in conjunction with 4800 MALDI TOF/TOF.
Amongst these we observed changes in βHCG, a known screening marker for DS, indicating that our assay was functional.
We found a number of elevated proteins Ig lambda chain C region, serum amyloid P-component, amyloid beta A4, and under expressed proteins like gamma-actin and titin in DS pregnancies.
These proteins are also found in the sera of patients with Alzheimer disease, which share similar pathologies of DS.
Our study therefore indicates that the iTRAQ labelling approach may be indeed useful for the detection of novel biomarkers.
American Psychological Association (APA)
Kolla, Varaprasad& Jenö, Paul& Moes, Suzette& Tercanli, Sevgi& Lapaire, Olav& Choolani, Mahesh…[et al.]. 2009. Quantitative Proteomics Analysis of Maternal Plasma in Down Syndrome Pregnancies Using Isobaric Tagging Reagent (iTRAQ). BioMed Research International،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-989203
Modern Language Association (MLA)
Kolla, Varaprasad…[et al.]. Quantitative Proteomics Analysis of Maternal Plasma in Down Syndrome Pregnancies Using Isobaric Tagging Reagent (iTRAQ). BioMed Research International No. 2010 (Dec. 2010), pp.1-10.
https://search.emarefa.net/detail/BIM-989203
American Medical Association (AMA)
Kolla, Varaprasad& Jenö, Paul& Moes, Suzette& Tercanli, Sevgi& Lapaire, Olav& Choolani, Mahesh…[et al.]. Quantitative Proteomics Analysis of Maternal Plasma in Down Syndrome Pregnancies Using Isobaric Tagging Reagent (iTRAQ). BioMed Research International. 2009. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-989203
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989203