Improvement of Capture Compound Mass Spectrometry Technology (CCMS) for the Profiling of Human Kinases by Combination with 2D LC-MSMS
المؤلفون المشاركون
Glinski, Mirko
Fischer, Jenny J.
Dreger, Mathias
Baumgart, Sabine
Koester, Hubert
Graebner, Olivia
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-09-19
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
An increasingly popular and promising field in functional proteomics is the isolation of proteome subsets based on small molecule-protein interactions.
One platform approach in this field are Capture Compounds that contain a small molecule of interest to bind target proteins, a photo-activatable reactivity function to covalently trap bound proteins, and a sorting function to isolate captured protein conjugates from complex biological samples for direct protein identification by liquid chromatography/mass spectrometry (nLC-MS/MS).
In this study we used staurosporine as a selectivity group for analysis in HepG2 cells derived from human liver.
In the present study, we combined the functional isolation of kinases with different separation workflows of automated split-free nanoflow liquid chromatography prior to mass spectrometric analysis.
Two different CCMS setups, CCMS technology combined with 1D LC-MS and 2D LC-MS, were compared regarding the total number of kinase identifications.
By extending the chromatographic separation of the tryptic digested captured proteins from 1D LC linear gradients to 2D LC we were able to identify 97 kinases.
This result is similar to the 1D LC setup we previously reported but this time 4 times less input material was needed.
This makes CCMS of kinases an even more powerful tool for the proteomic profiling of this important protein family.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fischer, Jenny J.& Graebner, Olivia& Dreger, Mathias& Glinski, Mirko& Baumgart, Sabine& Koester, Hubert. 2011. Improvement of Capture Compound Mass Spectrometry Technology (CCMS) for the Profiling of Human Kinases by Combination with 2D LC-MSMS. BioMed Research International،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-990342
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fischer, Jenny J.…[et al.]. Improvement of Capture Compound Mass Spectrometry Technology (CCMS) for the Profiling of Human Kinases by Combination with 2D LC-MSMS. BioMed Research International No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-990342
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fischer, Jenny J.& Graebner, Olivia& Dreger, Mathias& Glinski, Mirko& Baumgart, Sabine& Koester, Hubert. Improvement of Capture Compound Mass Spectrometry Technology (CCMS) for the Profiling of Human Kinases by Combination with 2D LC-MSMS. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-990342
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-990342
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر