Improvement of Capture Compound Mass Spectrometry Technology (CCMS) for the Profiling of Human Kinases by Combination with 2D LC-MSMS
Joint Authors
Glinski, Mirko
Fischer, Jenny J.
Dreger, Mathias
Baumgart, Sabine
Koester, Hubert
Graebner, Olivia
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
An increasingly popular and promising field in functional proteomics is the isolation of proteome subsets based on small molecule-protein interactions.
One platform approach in this field are Capture Compounds that contain a small molecule of interest to bind target proteins, a photo-activatable reactivity function to covalently trap bound proteins, and a sorting function to isolate captured protein conjugates from complex biological samples for direct protein identification by liquid chromatography/mass spectrometry (nLC-MS/MS).
In this study we used staurosporine as a selectivity group for analysis in HepG2 cells derived from human liver.
In the present study, we combined the functional isolation of kinases with different separation workflows of automated split-free nanoflow liquid chromatography prior to mass spectrometric analysis.
Two different CCMS setups, CCMS technology combined with 1D LC-MS and 2D LC-MS, were compared regarding the total number of kinase identifications.
By extending the chromatographic separation of the tryptic digested captured proteins from 1D LC linear gradients to 2D LC we were able to identify 97 kinases.
This result is similar to the 1D LC setup we previously reported but this time 4 times less input material was needed.
This makes CCMS of kinases an even more powerful tool for the proteomic profiling of this important protein family.
American Psychological Association (APA)
Fischer, Jenny J.& Graebner, Olivia& Dreger, Mathias& Glinski, Mirko& Baumgart, Sabine& Koester, Hubert. 2011. Improvement of Capture Compound Mass Spectrometry Technology (CCMS) for the Profiling of Human Kinases by Combination with 2D LC-MSMS. BioMed Research International،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-990342
Modern Language Association (MLA)
Fischer, Jenny J.…[et al.]. Improvement of Capture Compound Mass Spectrometry Technology (CCMS) for the Profiling of Human Kinases by Combination with 2D LC-MSMS. BioMed Research International No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-990342
American Medical Association (AMA)
Fischer, Jenny J.& Graebner, Olivia& Dreger, Mathias& Glinski, Mirko& Baumgart, Sabine& Koester, Hubert. Improvement of Capture Compound Mass Spectrometry Technology (CCMS) for the Profiling of Human Kinases by Combination with 2D LC-MSMS. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-990342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990342