Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus in Dairy Cow Suffering from Mastitis

المؤلفون المشاركون

Chunguang, Wang
Zhang, Tie
Xiuhui, Zhong
Zhao, Xinghua
Wang, Chunguang

المصدر

BioMed Research International

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-11-19

دولة النشر

مصر

عدد الصفحات

5

التخصصات الرئيسية

الطب البشري

الملخص EN

To develop a rapid detection method of Staphylococcus aureus using loop-mediated isothermal amplification (LAMP), four specific primers were designed according to six distinct sequences of the nuc gene.

In addition, the specificity and sensitivity of LAMP were verified and compared with those of PCR.

Results showed that the LAMP reaction was completed within 45 min at 62.5°C, and ladder bands were appeared in LAMP products analyzed by gel electrophoresis.

After adding 1x SYBR Green l, the positive reaction tube showed green color and the negative reaction tube remained orange, indicating that the LAMP has high specificity.

The minimal detectable concentration of LAMP was 1×102 CFU/mL and that of PCR was 1×104 CFU/mL, indicating that the LAMP was 100 times more sensitive than the PCR.

The LAMP method for detection of Staphylococcus aureus has many advantages, such as simple operation, high sensitivity, high specificity, and rapid analysis.

Therefore, this method is more suitable for the rapid on-site detection of Staphylococcus aureus.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zhang, Tie& Chunguang, Wang& Wang, Chunguang& Zhao, Xinghua& Xiuhui, Zhong. 2012. Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus in Dairy Cow Suffering from Mastitis. BioMed Research International،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-991702

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zhang, Tie…[et al.]. Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus in Dairy Cow Suffering from Mastitis. BioMed Research International No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-991702

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zhang, Tie& Chunguang, Wang& Wang, Chunguang& Zhao, Xinghua& Xiuhui, Zhong. Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus in Dairy Cow Suffering from Mastitis. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-991702

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-991702