Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus in Dairy Cow Suffering from Mastitis
Joint Authors
Chunguang, Wang
Zhang, Tie
Xiuhui, Zhong
Zhao, Xinghua
Wang, Chunguang
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
To develop a rapid detection method of Staphylococcus aureus using loop-mediated isothermal amplification (LAMP), four specific primers were designed according to six distinct sequences of the nuc gene.
In addition, the specificity and sensitivity of LAMP were verified and compared with those of PCR.
Results showed that the LAMP reaction was completed within 45 min at 62.5°C, and ladder bands were appeared in LAMP products analyzed by gel electrophoresis.
After adding 1x SYBR Green l, the positive reaction tube showed green color and the negative reaction tube remained orange, indicating that the LAMP has high specificity.
The minimal detectable concentration of LAMP was 1×102 CFU/mL and that of PCR was 1×104 CFU/mL, indicating that the LAMP was 100 times more sensitive than the PCR.
The LAMP method for detection of Staphylococcus aureus has many advantages, such as simple operation, high sensitivity, high specificity, and rapid analysis.
Therefore, this method is more suitable for the rapid on-site detection of Staphylococcus aureus.
American Psychological Association (APA)
Zhang, Tie& Chunguang, Wang& Wang, Chunguang& Zhao, Xinghua& Xiuhui, Zhong. 2012. Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus in Dairy Cow Suffering from Mastitis. BioMed Research International،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-991702
Modern Language Association (MLA)
Zhang, Tie…[et al.]. Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus in Dairy Cow Suffering from Mastitis. BioMed Research International No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-991702
American Medical Association (AMA)
Zhang, Tie& Chunguang, Wang& Wang, Chunguang& Zhao, Xinghua& Xiuhui, Zhong. Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus in Dairy Cow Suffering from Mastitis. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-991702
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991702