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Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size
المؤلفون المشاركون
Antosik, Paweł
Nowicki, Michał
Woźna, Magdalena
Jackowska, Marta
Piotrowska, Hanna
Jaśkowski, Jędrzej M.
Zawierucha, Piotr
Bukowska, Dorota
Brüssow, Klaus P.
Kempisty, Bartosz
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-20
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Cumulus-oocyte-complexes (COCs) were collected from small (<3 mm), medium (3–5 mm), and large (>5 mm) porcine follicles, and the INHA and INHB expression and cellular localization were studied.
Developmentally competent (BCB+) COCs were cultured for 44 h.
Samples of mRNA were isolated before and after in vitro maturation (IVM) from oocytes collected from follicles of different size for RQ-PCR assay.
The INHA and INHB protein distribution within the oocytes was observed by confocal microscopy.
INHA mRNA expression was increased in oocytes from large compared to medium and small follicles before IVM (P<0.001), and to oocytes of small follicles after IVM (P<0.001).
The INHB expression was not different before IVM, but the IHNB mRNA level was gradually higher in oocytes from large follicles after IVM (P<0.01).
INHA was not differently expressed before IVM; however, in large follicle oocytes the protein was distributed in the peripheral area of the cytoplasm; in oocytes from small follicles it was in the entire cytoplasm.
After IVM, INHA was strongly expressed in oocytes from small follicles and distributed particularly in the zona pellucida (ZP).
Similarly and both before and after IVM, INHB protein was highly expressed in small follicle oocytes and within the cytoplasm.
In summary, INHs can be recognized as a marker of porcine oocyte quality.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kempisty, Bartosz& Jackowska, Marta& Woźna, Magdalena& Antosik, Paweł& Piotrowska, Hanna& Zawierucha, Piotr…[et al.]. 2012. Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991872
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kempisty, Bartosz…[et al.]. Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991872
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kempisty, Bartosz& Jackowska, Marta& Woźna, Magdalena& Antosik, Paweł& Piotrowska, Hanna& Zawierucha, Piotr…[et al.]. Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991872
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-991872
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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