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Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size
Joint Authors
Antosik, Paweł
Nowicki, Michał
Woźna, Magdalena
Jackowska, Marta
Piotrowska, Hanna
Jaśkowski, Jędrzej M.
Zawierucha, Piotr
Bukowska, Dorota
Brüssow, Klaus P.
Kempisty, Bartosz
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Cumulus-oocyte-complexes (COCs) were collected from small (<3 mm), medium (3–5 mm), and large (>5 mm) porcine follicles, and the INHA and INHB expression and cellular localization were studied.
Developmentally competent (BCB+) COCs were cultured for 44 h.
Samples of mRNA were isolated before and after in vitro maturation (IVM) from oocytes collected from follicles of different size for RQ-PCR assay.
The INHA and INHB protein distribution within the oocytes was observed by confocal microscopy.
INHA mRNA expression was increased in oocytes from large compared to medium and small follicles before IVM (P<0.001), and to oocytes of small follicles after IVM (P<0.001).
The INHB expression was not different before IVM, but the IHNB mRNA level was gradually higher in oocytes from large follicles after IVM (P<0.01).
INHA was not differently expressed before IVM; however, in large follicle oocytes the protein was distributed in the peripheral area of the cytoplasm; in oocytes from small follicles it was in the entire cytoplasm.
After IVM, INHA was strongly expressed in oocytes from small follicles and distributed particularly in the zona pellucida (ZP).
Similarly and both before and after IVM, INHB protein was highly expressed in small follicle oocytes and within the cytoplasm.
In summary, INHs can be recognized as a marker of porcine oocyte quality.
American Psychological Association (APA)
Kempisty, Bartosz& Jackowska, Marta& Woźna, Magdalena& Antosik, Paweł& Piotrowska, Hanna& Zawierucha, Piotr…[et al.]. 2012. Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991872
Modern Language Association (MLA)
Kempisty, Bartosz…[et al.]. Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991872
American Medical Association (AMA)
Kempisty, Bartosz& Jackowska, Marta& Woźna, Magdalena& Antosik, Paweł& Piotrowska, Hanna& Zawierucha, Piotr…[et al.]. Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991872
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991872