Ecklonia cava Inhibits Glucose Absorption and Stimulates Insulin Secretion in Streptozotocin-Induced Diabetic Mice

الملخص EN

Aims of study.

Present study investigated the effect of Ecklonia cava (EC) on intestinal glucose uptake and insulin secretion.

Materials and methods.

Intestinal Na+-dependent glucose uptake (SGU) and Na+-dependent glucose transporter 1 (SGLT1) protein expression was determined using brush border membrane vesicles (BBMVs).

Glucose-induced insulin secretion was examined in pancreatic β-islet cells.

The antihyperglycemic effects of EC, SGU, and SGLT1 expression were determined in streptozotocin (STZ)-induced diabetic mice.

Results.

Methanol extract of EC markedly inhibited intestinal SGU of BBMV with the IC50 value of 345 μg/mL.

SGLT1 protein expression was dose dependently down regulated with EC treatment.

Furthermore, insulinotrophic effect of EC extract was observed at high glucose media in isolated pancreatic β-islet cells in vitro.

We next conducted the antihyperglycemic effect of EC in STZ-diabetic mice.

EC supplementation markedly suppressed SGU and SGLT1 abundance in BBMV from STZ mice.

Furthermore, plasma insulin level was increased by EC treatment in diabetic mice.

As a result, EC supplementation improved postprandial glucose regulation, assessed by oral glucose tolerance test, in diabetic mice.

Conclusion.

These results suggest that EC play a role in controlling dietary glucose absorption at the intestine and insulinotrophic action at the pancreas contributing blood glucose homeostasis in diabetic condition.