Extraction, partial purification and some factors affecting milk clotting activity of a milk coagulant prepared from sunflower (Helianthus annus) seeds
Other Title(s)
الاستخلاص، التنقية الجزئية و بعض العوامل المؤثرة على القدرة التجبنية لمستحضر أنزيمي مجبن للبن من بذور دوار الشمس
Joint Authors
Abd al-Baqi, Atiyyah Abd al-Muti
Abd al-Wahid, al-Sayyid Muhammad
Rabi, Abd al-Hamid Muhammad Hasan
Ramadan, Islam Ibrahim
Source
Zagazig Journal of Agricultural Research
Issue
Vol. 46, Issue 2 (30 Apr. 2019), pp.441-453, 13 p.
Publisher
Zagazig University Faculty of Agriculture
Publication Date
2019-04-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Topics
Abstract EN
Crude extract of sunflower seeds was prepared using different extractants (distilled
water, 5% NaCl in distilled water, 5% NaCl in acetate buffer pH 5.0) and certain mixing ratios (1: 2,
1: 5 and 1: 10 seeds powder: extractant, respectively).
Using of 5% NaCl in acetate buffer pH 5.0 at
mixing ratio of 1: 5 gave an extract with reasonable efficient clotting properties.
Ammonium sulphate
at 40-60% concentration was used in one single step for the partial purification of the extract.
Ammonium sulphate, (40-60% ) fraction showed milk clotting and specific activities of 440 sec and
2.30 U/mg protein, respectively.
Meanwhile a yield percentage and a fold of purification of 28.58 and
2.02 were achieved.
Clotting/proteolytic activity, water holding capacity and curd synersis of the
partially purified extract approved those of commercial calf rennet indicating the suitability of the
extract as a rennet substitute.
The optimum temperature, pH, Cacl2 and GDL concentration of the
resultant extracts were found to be 65°C, 5.0, 0.04 % and 0.4% , respectively.
American Psychological Association (APA)
Ramadan, Islam Ibrahim& Abd al-Wahid, al-Sayyid Muhammad& Rabi, Abd al-Hamid Muhammad Hasan& Abd al-Baqi, Atiyyah Abd al-Muti. 2019. Extraction, partial purification and some factors affecting milk clotting activity of a milk coagulant prepared from sunflower (Helianthus annus) seeds. Zagazig Journal of Agricultural Research،Vol. 46, no. 2, pp.441-453.
https://search.emarefa.net/detail/BIM-1023805
Modern Language Association (MLA)
Abd al-Baqi, Atiyyah Abd al-Muti…[et al.]. Extraction, partial purification and some factors affecting milk clotting activity of a milk coagulant prepared from sunflower (Helianthus annus) seeds. Zagazig Journal of Agricultural Research Vol. 46, no. 2 (2019), pp.441-453.
https://search.emarefa.net/detail/BIM-1023805
American Medical Association (AMA)
Ramadan, Islam Ibrahim& Abd al-Wahid, al-Sayyid Muhammad& Rabi, Abd al-Hamid Muhammad Hasan& Abd al-Baqi, Atiyyah Abd al-Muti. Extraction, partial purification and some factors affecting milk clotting activity of a milk coagulant prepared from sunflower (Helianthus annus) seeds. Zagazig Journal of Agricultural Research. 2019. Vol. 46, no. 2, pp.441-453.
https://search.emarefa.net/detail/BIM-1023805
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1023805