Nestin Protein Is Phosphorylated in Adult Neural StemProgenitor Cells and Not Endothelial Progenitor Cells
Joint Authors
Namiki, Jun
Suzuki, Sayuri
Masuda, Takeshi
Ishihama, Yasushi
Okano, Hideyuki
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-13
Country of Publication
Egypt
No. of Pages
5
Abstract EN
An intermediate filament protein, Nestin, is known as a neural stem/progenitor cell marker.
It was shown to be required for the survival and self-renewal of neural stem cells according to the phenotypes of Nestin knockout mice.
Nestin expression has also been reported in vascular endothelial cells, and we recently reported Nestin expression in proliferating endothelial progenitor cells, but not in mature endothelial cells.
Using quantitative phosphoproteome analysis, we studied differences in phosphorylation levels between CNS Nestin in adult neural stem cells and vascular Nestin in adult bone-marrow-derived endothelial progenitor cells.
We detected 495 phosphopeptides in the cell lysates of adult CNS stem/progenitor cells and identified 11 significant phosphorylated amino acid residues in the Nestin protein.
In contrast, endothelial progenitor cells showed no significant phosphorylation of Nestin.
We also measured neoplastic endothelial cells of the mouse brain and identified 13 phosphorylated amino acid residues in the Nestin protein.
Among the 11 phosphorylated amino acids of adult CNS Nestin, five (S565, S570, S819, S883, and S886) were CNS Nestin-specific phosphorylation sites.
Detection of the CNS-specific phosphorylation sites in Nestin, for example, by a phospho-specific Nestin antibody, may allow the expression of CNS Nestin to be distinguished from vascular Nestin.
American Psychological Association (APA)
Namiki, Jun& Suzuki, Sayuri& Masuda, Takeshi& Ishihama, Yasushi& Okano, Hideyuki. 2012. Nestin Protein Is Phosphorylated in Adult Neural StemProgenitor Cells and Not Endothelial Progenitor Cells. Stem Cells International،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1003010
Modern Language Association (MLA)
Namiki, Jun…[et al.]. Nestin Protein Is Phosphorylated in Adult Neural StemProgenitor Cells and Not Endothelial Progenitor Cells. Stem Cells International No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-1003010
American Medical Association (AMA)
Namiki, Jun& Suzuki, Sayuri& Masuda, Takeshi& Ishihama, Yasushi& Okano, Hideyuki. Nestin Protein Is Phosphorylated in Adult Neural StemProgenitor Cells and Not Endothelial Progenitor Cells. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1003010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003010