Nestin Protein Is Phosphorylated in Adult Neural StemProgenitor Cells and Not Endothelial Progenitor Cells
المؤلفون المشاركون
Namiki, Jun
Suzuki, Sayuri
Masuda, Takeshi
Ishihama, Yasushi
Okano, Hideyuki
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-09-13
دولة النشر
مصر
عدد الصفحات
5
الملخص EN
An intermediate filament protein, Nestin, is known as a neural stem/progenitor cell marker.
It was shown to be required for the survival and self-renewal of neural stem cells according to the phenotypes of Nestin knockout mice.
Nestin expression has also been reported in vascular endothelial cells, and we recently reported Nestin expression in proliferating endothelial progenitor cells, but not in mature endothelial cells.
Using quantitative phosphoproteome analysis, we studied differences in phosphorylation levels between CNS Nestin in adult neural stem cells and vascular Nestin in adult bone-marrow-derived endothelial progenitor cells.
We detected 495 phosphopeptides in the cell lysates of adult CNS stem/progenitor cells and identified 11 significant phosphorylated amino acid residues in the Nestin protein.
In contrast, endothelial progenitor cells showed no significant phosphorylation of Nestin.
We also measured neoplastic endothelial cells of the mouse brain and identified 13 phosphorylated amino acid residues in the Nestin protein.
Among the 11 phosphorylated amino acids of adult CNS Nestin, five (S565, S570, S819, S883, and S886) were CNS Nestin-specific phosphorylation sites.
Detection of the CNS-specific phosphorylation sites in Nestin, for example, by a phospho-specific Nestin antibody, may allow the expression of CNS Nestin to be distinguished from vascular Nestin.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Namiki, Jun& Suzuki, Sayuri& Masuda, Takeshi& Ishihama, Yasushi& Okano, Hideyuki. 2012. Nestin Protein Is Phosphorylated in Adult Neural StemProgenitor Cells and Not Endothelial Progenitor Cells. Stem Cells International،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1003010
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Namiki, Jun…[et al.]. Nestin Protein Is Phosphorylated in Adult Neural StemProgenitor Cells and Not Endothelial Progenitor Cells. Stem Cells International No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-1003010
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Namiki, Jun& Suzuki, Sayuri& Masuda, Takeshi& Ishihama, Yasushi& Okano, Hideyuki. Nestin Protein Is Phosphorylated in Adult Neural StemProgenitor Cells and Not Endothelial Progenitor Cells. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1003010
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003010
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر