Stimulation of Cultured H9 Human Embryonic Stem Cells with Thyroid Stimulating Hormone Does Not Lead to Formation of Thyroid-Like Cells
Joint Authors
Neumann, Ronald D.
Onyshchenko, Mykola I.
Panyutin, Igor G.
Panyutin, Irina V.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-11
Country of Publication
Egypt
No. of Pages
8
Abstract EN
The sodium-iodine symporter (NIS) is expressed on the cell membrane of many thyroid cancer cells, and is responsible for the radioactive iodine accumulation.
However, treatment of anaplastic thyroid cancer is ineffective due to the low expression of NIS on cell membranes of these tumor cells.
Human embryonic stem cells (ESCs) provide a potential vehicle to study the mechanisms of NIS expression regulation during differentiation.
Human ESCs were maintained on feeder-independent culture conditions.
RT-qPCR and immunocytochemistry were used to study differentiation marker expression, 125I uptake to study NIS function.
We designed a two-step protocol for human ESC differentiation into thyroid-like cells, as was previously done for mouse embryonic stem cells.
First, we obtained definitive endoderm from human ESCs.
Second, we directed differentiation of definitive endoderm cells into thyroid-like cells using various factors, with thyroid stimulating hormone (TSH) as the main differentiating factor.
Expression of pluripotency, endoderm and thyroid markers and 125I uptake were monitored throughout the differentiation steps.
These approaches did not result in efficient induction of thyroid-like cells.
We conclude that differentiation of human ESCs into thyroid cells cannot be induced by TSH media supplementation alone and most likely involves complicated developmental patterns that are yet to be understood.
American Psychological Association (APA)
Onyshchenko, Mykola I.& Panyutin, Igor G.& Panyutin, Irina V.& Neumann, Ronald D.. 2012. Stimulation of Cultured H9 Human Embryonic Stem Cells with Thyroid Stimulating Hormone Does Not Lead to Formation of Thyroid-Like Cells. Stem Cells International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1003063
Modern Language Association (MLA)
Onyshchenko, Mykola I.…[et al.]. Stimulation of Cultured H9 Human Embryonic Stem Cells with Thyroid Stimulating Hormone Does Not Lead to Formation of Thyroid-Like Cells. Stem Cells International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-1003063
American Medical Association (AMA)
Onyshchenko, Mykola I.& Panyutin, Igor G.& Panyutin, Irina V.& Neumann, Ronald D.. Stimulation of Cultured H9 Human Embryonic Stem Cells with Thyroid Stimulating Hormone Does Not Lead to Formation of Thyroid-Like Cells. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1003063
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003063