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Characterization and Application of Enterocin RM6, a Bacteriocin from Enterococcus faecalis
Joint Authors
Huang, En
Zhang, Liwen
Chung, Yoon-Kyung
Zheng, Zuoxing
Yousef, Ahmed E.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Use of bacteriocins in food preservation has received great attention in recent years.
The goal of this study is to characterize enterocin RM6 from Enterococcus faecalis OSY-RM6 and investigate its efficacy against Listeria monocytogenes in cottage cheese.
Enterocin RM6 was purified from E.
faecalis culture supernatant using ion exchange column, multiple C18-silica cartridges, followed by reverse-phase high-performance liquid chromatography.
The molecular weight of enterocin RM6 is 7145.0823 as determined by mass spectrometry (MS).
Tandem mass spectrometry (MS/MS) analysis revealed that enterocin RM6 is a 70-residue cyclic peptide with a head-to-tail linkage between methionine and tryptophan residues.
The peptide sequence of enterocin RM6 was further confirmed by sequencing the structural gene of the peptide.
Enterocin RM6 is active against Gram-positive bacteria, including L.
monocytogenes, Bacillus cereus, and methicillin-resistant Staphylococcus aureus (MRSA).
Enterocin RM6 (final concentration in cottage cheese, 80 AU/mL) caused a 4-log reduction in population of L.
monocytogenes inoculated in cottage cheese within 30 min of treatment.
Therefore, enterocin RM6 has potential applications as a potent antimicrobial peptide against foodborne pathogens in food.
American Psychological Association (APA)
Huang, En& Zhang, Liwen& Chung, Yoon-Kyung& Zheng, Zuoxing& Yousef, Ahmed E.. 2013. Characterization and Application of Enterocin RM6, a Bacteriocin from Enterococcus faecalis. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003707
Modern Language Association (MLA)
Huang, En…[et al.]. Characterization and Application of Enterocin RM6, a Bacteriocin from Enterococcus faecalis. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1003707
American Medical Association (AMA)
Huang, En& Zhang, Liwen& Chung, Yoon-Kyung& Zheng, Zuoxing& Yousef, Ahmed E.. Characterization and Application of Enterocin RM6, a Bacteriocin from Enterococcus faecalis. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003707
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003707