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Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease
Joint Authors
Zheng, Yanbin
Chen, Qiushi
Shan, Anshan
Zhang, Hao
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
For utilizing the blood cells (BCs) effectively, enzymatic hydrolysis was applied to produce the enzymatically hydrolyzed blood cells (EHBCs) by using a neutral protease as a catalyst.
The results of the single-factor experiments showed optimal substrate concentration, enzyme to substrate ratio (E/S), pH, temperature, and incubation period were 1.00%, 0.10, 7.00, 50.00°C, and 12.00 h, respectively.
The optimized hydrolysis conditions from response surface methodology (RSM) were pH 6.50, E/S 0.11, temperature 45.00°C, and incubation period 12.00 h.
Under these conditions (substrate concentration 1.00%), the degree of hydrolysis (DH) was 35.06%.
The free amino acids (FAAs) content of the EHBCs (35.24%) was 40.46 times higher than BCs while the total amino acids (TAAs) content was lower than BCs.
The scores of lysine (human 0.87; pig 0.97), valine (human 1.42; pig 1.38), leucine (human 1.50; pig 1.90), tyrosine (human 0.84; pig 1.09), and histidine (human 2.17; pig 2.50) indicated that the EHBCs basically fulfilled the adult human and pig nutritional requirements.
The calculated protein efficiency ratios (C-PERs) of the EHBCs were 3.94, 6.19, 21.73, and 2.04.
In summary, the EHBCs were produced successfully with optimized conditions and could be a novel protein source for humans and pigs.
American Psychological Association (APA)
Zheng, Yanbin& Chen, Qiushi& Shan, Anshan& Zhang, Hao. 2012. Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003869
Modern Language Association (MLA)
Zheng, Yanbin…[et al.]. Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1003869
American Medical Association (AMA)
Zheng, Yanbin& Chen, Qiushi& Shan, Anshan& Zhang, Hao. Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003869
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003869