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Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure
Joint Authors
Kiczak, Liliana
Tomaszek, Alicja
Bania, Jacek
Paslawska, Urszula
Zacharski, Maciej
Noszczyk-Nowak, Agnieszka
Janiszewski, Adrian
Skrzypczak, Piotr
Ardehali, Hossein
Jankowska, Ewa A.
Ponikowski, Piotr
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Matrix metalloproteinases (MMPs) are involved in the remodeling of extracellular matrix in various tissues.
Their functioning could be related to the formation of complexes, containing MMP9, MMP2, tissue inhibitor of metalloproteinases type 1 (TIMP1), and neutrophil gelatinase-associated lipocalin (NGAL).
Such complexes have not been investigated in either myocardial or skeletal muscles.
We examined 20 male pigs with heart failure (HF), and 5 sham-operated animals.
There were no differences in the mRNA expression of MMP9, MMP2, TIMP1, and NGAL between diseased and healthy animals, in either left ventricle (LV) myocardium or skeletal muscles.
In LV from both diseased and healthy animals, in nonreducing and nondenaturing conditions, we demonstrated the presence of high molecular weight (HMW) complexes (130, 170, and 220 kDa) containing MMP9, TIMP1, and NGAL (also MMP2 in 220 kDa complex) without proteolytic activity, and a proteolytically active 115 kDa MMP9 form together with 72 and 68 kDa bands (proMMP2 and MMP2).
Proteolytically active bands were also spontaneously released from HMW complexes.
In skeletal muscles from both diseased and healthy animals, in nonreducing and nondenaturing conditions, we found no HMW complexes, and proteolytic activity was associated with the presence of 72 and 68 kDa bands (proMMP2 and MMP2).
American Psychological Association (APA)
Kiczak, Liliana& Tomaszek, Alicja& Bania, Jacek& Paslawska, Urszula& Zacharski, Maciej& Noszczyk-Nowak, Agnieszka…[et al.]. 2013. Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1003889
Modern Language Association (MLA)
Kiczak, Liliana…[et al.]. Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1003889
American Medical Association (AMA)
Kiczak, Liliana& Tomaszek, Alicja& Bania, Jacek& Paslawska, Urszula& Zacharski, Maciej& Noszczyk-Nowak, Agnieszka…[et al.]. Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1003889
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003889