Effect of Intermittent Low-Frequency Electrical Stimulation on the Rat Gastrocnemius Muscle
Joint Authors
Tsutaki, Arata
Ogasawara, Riki
Kobayashi, Koji
Lee, Kihyuk
Kouzaki, Karina
Nakazato, Koichi
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Low-frequency neuromuscular electrical stimulation (NMES) has been used as an endurance exercise model.
This study aimed to test whether low-frequency NMES increases the phosphorylation of anabolic signaling molecules and induces skeletal muscle hypertrophy, as seen with high-frequency NMES.
Using Sprague-Dawley rats, 1 bout of exercise (with dissection done immediately (Post0) and 3 h (Post3) after exercise) and another 6 sessions of training were performed.
All experimental groups consisted of high- and low-frequency stimulation (HFS: 100 Hz; LFS: 10 Hz).
Periodic acid-Schiff (PAS) staining was conducted to investigate type II fiber activation, and western blot analysis (WB) was conducted to examine whether NMES leads to anabolic intracellular signaling.
At first, we examined the acute effect of exercise.
PAS staining revealed that glycogen depletion occurred in both type I and type II fibers.
WB results demonstrated that p70S6K phosphorylation was significantly increased by HFS, but there was no significant difference with LFS.
In contrast, ERK 1/2 phosphorylation was increased by LFS at Post0.
In the 6-session training, the wet weight and myofibrillar protein were significantly increased by both HFS and LFS.
In conclusion, LFS has a similar anabolic effect for skeletal muscle hypertrophy as HFS, but the mediating signaling pathway might differ.
American Psychological Association (APA)
Tsutaki, Arata& Ogasawara, Riki& Kobayashi, Koji& Lee, Kihyuk& Kouzaki, Karina& Nakazato, Koichi. 2013. Effect of Intermittent Low-Frequency Electrical Stimulation on the Rat Gastrocnemius Muscle. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004409
Modern Language Association (MLA)
Tsutaki, Arata…[et al.]. Effect of Intermittent Low-Frequency Electrical Stimulation on the Rat Gastrocnemius Muscle. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1004409
American Medical Association (AMA)
Tsutaki, Arata& Ogasawara, Riki& Kobayashi, Koji& Lee, Kihyuk& Kouzaki, Karina& Nakazato, Koichi. Effect of Intermittent Low-Frequency Electrical Stimulation on the Rat Gastrocnemius Muscle. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004409
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004409