Whole Blood Platelet Aggregation and Release Reaction Testing in Uremic Patients
Joint Authors
Zeck, Jay
Schallheim, Jason
Lew, Susie Q.
DePalma, Louis
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-26
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Platelet function analysis utilizing platelet-rich plasma and optical density based aggregometry fails to identify patients at risk for uremia associated complications.
Methods.
We employed whole blood platelet aggregation analysis based on impedance as well as determination of ATP release from platelet granules detected by a chemiluminescence method.
Ten chronic kidney disease (CKD) stage 4 or 5 predialysis patients underwent platelet evaluation.
Our study aims to evaluate this platform in this patient population to determine if abnormalities could be detected.
Results.
Analysis revealed normal aggregation and ATP release to collagen, ADP, and high-dose ristocetin.
ATP release had a low response to arachidonic acid (0.37 ± 0.26 nmoles, reference range: 0.6–1.4 nmoles).
Platelet aggregation to low-dose ristocetin revealed an exaggerated response (20.9 ± 18.7 ohms, reference range: 0–5 ohms).
Conclusions.
Whole blood platelet analysis detected platelet dysfunction which may be associated with bleeding and thrombotic risks in uremia.
Diminished ATP release to arachidonic acid (an aspirin-like defect) in uremic patients may result in platelet associated bleeding.
An increased aggregation response to low-dose ristocetin (a type IIb von Willebrand disease-like defect) is associated with thrombus formation.
This platelet hyperreactivity may be associated with a thrombotic diathesis as seen in some uremic patients.
American Psychological Association (APA)
Zeck, Jay& Schallheim, Jason& Lew, Susie Q.& DePalma, Louis. 2013. Whole Blood Platelet Aggregation and Release Reaction Testing in Uremic Patients. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004431
Modern Language Association (MLA)
Zeck, Jay…[et al.]. Whole Blood Platelet Aggregation and Release Reaction Testing in Uremic Patients. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1004431
American Medical Association (AMA)
Zeck, Jay& Schallheim, Jason& Lew, Susie Q.& DePalma, Louis. Whole Blood Platelet Aggregation and Release Reaction Testing in Uremic Patients. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004431
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004431