SeedSeq: Off-Target Transcriptome Database
Joint Authors
Kozak, Karol
Das, Shaoli
Ghosal, Suman
Chakrabarti, Jayprokas
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Detection of potential cross-reaction between a short oligonucleotide sequence and a longer (unintended) sequence is crucial for many biological applications, such as high content screening (HCS), microarray nucleotide probes, or short interfering RNAs (siRNAs).
However, owing to a tolerance for mismatches and gaps in base-pairing with target transcripts, siRNAs could have up to hundreds of potential target sequences in a genome, and some small RNAs in mammalian systems have been shown to affect the levels of many messenger RNAs (off-targets) besides their intended target transcripts (on-targets).
The reference sequence (RefSeq) collection aims to provide a comprehensive, integrated, nonredundant, well-annotated set of sequences, including mRNA transcripts.
We performed a detailed off-target analysis of three most commonly used kinome siRNA libraries based on the latest RefSeq version.
To simplify the access to off-target transcripts, we created a SeedSeq database, a new unique format to store off-target information.
American Psychological Association (APA)
Das, Shaoli& Ghosal, Suman& Chakrabarti, Jayprokas& Kozak, Karol. 2013. SeedSeq: Off-Target Transcriptome Database. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1005375
Modern Language Association (MLA)
Das, Shaoli…[et al.]. SeedSeq: Off-Target Transcriptome Database. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1005375
American Medical Association (AMA)
Das, Shaoli& Ghosal, Suman& Chakrabarti, Jayprokas& Kozak, Karol. SeedSeq: Off-Target Transcriptome Database. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1005375
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005375