BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage
Joint Authors
Jiao, Yu
Palmgren, Björn
Novozhilova, Ekaterina
Englund Johansson, Ulrica
Spieles-Engemann, Anne L.
Kale, Ajay
Stupp, Samuel I.
Olivius, Petri
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
|Objectives.
To study possible nerve regeneration of a damaged auditory nerve by the use of stem cell transplantation.
Methods.
We transplanted HNPCs to the rat AN trunk by the internal auditory meatus (IAM).
Furthermore, we studied if addition of BDNF affects survival and phenotypic differentiation of the grafted HNPCs.
A bioactive nanofiber gel (PA gel), in selected groups mixed with BDNF, was applied close to the implanted cells.
Before transplantation, all rats had been deafened by a round window niche application of β-bungarotoxin.
This neurotoxin causes a selective toxic destruction of the AN while keeping the hair cells intact.
Results.
Overall, HNPCs survived well for up to six weeks in all groups.
However, transplants receiving the BDNF-containing PA gel demonstrated significantly higher numbers of HNPCs and neuronal differentiation.
At six weeks, a majority of the HNPCs had migrated into the brain stem and differentiated.
Differentiated human cells as well as neurites were observed in the vicinity of the cochlear nucleus.
Conclusion.
Our results indicate that human neural precursor cells (HNPC) integration with host tissue benefits from additional brain derived neurotrophic factor (BDNF) treatment and that these cells appear to be good candidates for further regenerative studies on the auditory nerve (AN).
American Psychological Association (APA)
Jiao, Yu& Palmgren, Björn& Novozhilova, Ekaterina& Englund Johansson, Ulrica& Spieles-Engemann, Anne L.& Kale, Ajay…[et al.]. 2014. BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1016245
Modern Language Association (MLA)
Jiao, Yu…[et al.]. BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1016245
American Medical Association (AMA)
Jiao, Yu& Palmgren, Björn& Novozhilova, Ekaterina& Englund Johansson, Ulrica& Spieles-Engemann, Anne L.& Kale, Ajay…[et al.]. BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1016245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016245