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GBE50 Attenuates Inflammatory Response by Inhibiting the p38 MAPK and NF- κB Pathways in LPS-Stimulated Microglial Cells
Joint Authors
He, Gai-ying
Yuan, Chong-gang
Hao, Li
Xu, Ying
Zhang, Zhi-xiong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Overactivated microglia contribute to a variety of pathological conditions in the central nervous system.
The major goal of the present study is to evaluate the potential suppressing effects of a new type of Ginko biloba extract, GBE50, on activated microglia which causes proinflammatory responses and to explore the underlying molecular mechanisms.
Murine BV2 microglia cells, with or without pretreatmentof GBE50 at various concentrations, were activated by incubation with lipopolysaccharide (LPS).
A series of biochemical and microscopic assays were performed to measure cell viability, cell morphology, release of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), and signal transduction via the p38 MAPK and nuclear factor-kappa B (NF-κB) p65 pathways.
We found that GBE50 pretreatment suppressed LPS-induced morphological changes in BV2 cells.
Moreover, GBE50 treatment significantly reduced the release of proinflammatory cytokines, TNF-α and IL-1β, and inhibited the associated signal transduction through the p38 MAPK and NF-κB p65 pathways.
These results demonstrated the anti-inflammatory effect of GBE50 on LPS-activated BV2 microglia cells, and indicated that GBE50 reduced the LPS-induced proinflammatory TNF-α and IL-1β release by inhibiting signal transduction through the NF-κB p65 and p38 MAPK pathways.
Our findings reveal, at least in part, the molecular basis underlying the anti-inflammatory effects of GBE50.
American Psychological Association (APA)
He, Gai-ying& Yuan, Chong-gang& Hao, Li& Xu, Ying& Zhang, Zhi-xiong. 2014. GBE50 Attenuates Inflammatory Response by Inhibiting the p38 MAPK and NF- κB Pathways in LPS-Stimulated Microglial Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1018396
Modern Language Association (MLA)
He, Gai-ying…[et al.]. GBE50 Attenuates Inflammatory Response by Inhibiting the p38 MAPK and NF- κB Pathways in LPS-Stimulated Microglial Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1018396
American Medical Association (AMA)
He, Gai-ying& Yuan, Chong-gang& Hao, Li& Xu, Ying& Zhang, Zhi-xiong. GBE50 Attenuates Inflammatory Response by Inhibiting the p38 MAPK and NF- κB Pathways in LPS-Stimulated Microglial Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1018396
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018396