The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration
Joint Authors
Huang, Guo-fu
Zou, Jing
Shi, Jing
Zhang, Dong-you
Pen, Hong-fen
Zhang, Qi
Gao, Yu
Wang, Bo-yi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The present study was aimed at determining if the electroacupuncture (EA) is able to protect degenerated disc in vivo.
New Zealand white rabbits (n=40) were used for the study.
The rabbits were randomly assigned to four groups.
EA intervention was applied to one of the four groups.
Magnetic resonance imaging and Pfirrmann’s classification were obtained for each group to evaluate EA treatment on the intervertebral disc degeneration.
Discs were analyzed using immunofluorescence for the labeling of collagens 1 and 2, bone morphogenetic protein-2 (BMP-2), matrix metalloproteinase-13 (MMP-13), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1).
For protein expression analysis, western blot was used for biglycan and decorin.
Outcomes indicated that EA intervention decreased the grades compared with the compressed disc.
Immunofluorescence analysis showed a significant increase of collagens 1 and 2, TIMP-1, and BMP-2 positive cells, in contrast to MMP-13 after EA treatment for 28 days.
The protein expression showed a sign of regeneration that decorin and biglycan were upregulated.
It was concluded that EA contributed to the extracellular matrix (ECM) anabolic processes and increased the ECM components.
MMPs and their inhibitors involved in the mechanism of EA intervention on ECM decreased disc.
It kept a dynamic balance between ECM synthesis and degradation.
American Psychological Association (APA)
Huang, Guo-fu& Zou, Jing& Shi, Jing& Zhang, Dong-you& Pen, Hong-fen& Zhang, Qi…[et al.]. 2014. The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1018982
Modern Language Association (MLA)
Huang, Guo-fu…[et al.]. The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1018982
American Medical Association (AMA)
Huang, Guo-fu& Zou, Jing& Shi, Jing& Zhang, Dong-you& Pen, Hong-fen& Zhang, Qi…[et al.]. The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1018982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018982