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Wen-Dan Decoction Improves Negative Emotions in Sleep-Deprived Rats by Regulating Orexin-A and Leptin Expression
Joint Authors
Wu, Fengzhi
Song, Yuehan
Li, Feng
He, Xin
Ma, Jie
Feng, Ting
Guan, Binghe
Wang, Liye
Li, Sinai
Liu, Xiaolan
Liu, Yan
Mao, Meng
Liu, Jing
Bai, Shijing
Song, Cai
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Wen-Dan Decoction (WDD), a formula of traditional Chinese medicine, has been clinically used for treating insomnia for approximately 800 years.
However, the therapeutic mechanisms of WDD remain unclear.
Orexin-A plays a key role in the sleep-wake cycle, while leptin function is opposite to orexin-A.
Thus, orexin-A and leptin may be important factors in sleep disorders.
In this study, 48 rats were divided into control, model, WDD-treated, and diazepam-treated groups.
The model of insomnia was produced by sleep deprivation (SD) for 14 days.
The expressions of orexin-A, leptin, and their receptors in blood serum, prefrontal cortex, and hypothalamus were detected by enzyme-linked immunosorbent assay, immunohistochemistry, and real time PCR.
Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times.
Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group.
mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus.
WDD treatment normalized the behavior and upregulated orexin-A, leptin, orexin receptor 1 and leptin receptor in brain.
The findings suggest that WDD treatment may regulate SD-induced negative emotions by regulating orexin-A and leptin expression.
American Psychological Association (APA)
Wu, Fengzhi& Song, Yuehan& Li, Feng& He, Xin& Ma, Jie& Feng, Ting…[et al.]. 2014. Wen-Dan Decoction Improves Negative Emotions in Sleep-Deprived Rats by Regulating Orexin-A and Leptin Expression. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1020362
Modern Language Association (MLA)
Wu, Fengzhi…[et al.]. Wen-Dan Decoction Improves Negative Emotions in Sleep-Deprived Rats by Regulating Orexin-A and Leptin Expression. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1020362
American Medical Association (AMA)
Wu, Fengzhi& Song, Yuehan& Li, Feng& He, Xin& Ma, Jie& Feng, Ting…[et al.]. Wen-Dan Decoction Improves Negative Emotions in Sleep-Deprived Rats by Regulating Orexin-A and Leptin Expression. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1020362
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1020362