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The Antitumor Activity of Antrodia camphorata in Melanoma Cells: Modulation of Wntβ-Catenin Signaling Pathways
Joint Authors
Chang, Hsueh-Wei
Hseu, You Cheng
Yang, Hsin-Ling
Tsou, Hsiao-Tung
Lin, Kai-Yuan
Kumar, K. J. Senthil
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-25
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Antrodia camphorata (AC) is well known in Taiwan as a traditional Chinese medicine.
The aim of this study was to investigate whether a fermented culture broth of AC could inhibit melanoma proliferation and progression via suppression of the Wnt/β-catenin signaling pathway.
In this study, we observed that AC treatment resulted in decreased cell viability and disturbed Wnt/β-catenin cascade in B16F10 and/or B16F1 melanoma cells.
This result was accompanied by a decrease in the expression of Wnt/β-catenin transcriptional targets, including c-Myc and survivin.
Furthermore, treatment of melanoma cells with AC resulted in a significant increase in apoptosis, which was associated with DNA fragmentation, cytochrome c release, caspase-9 and -3 activation, PARP degradation, Bcl-2/Bax dysregulation, and p53 expression.
We also observed that AC caused G1 phase arrest mediated by a downregulation of cyclin D1 and CDK4 and increased p21 and p27 expression.
In addition, we demonstrated that non- and subcytotoxic concentrations of AC markedly inhibited migration and invasion of highly metastatic B16F10 cells.
The antimetastatic effect of AC was further confirmed by reductions in the levels of MMP-2, MMP-9, and VEGF expression.
These results suggest that Antrodia camphorata may exert antitumor activity by downregulating the Wnt/β-catenin pathways.
American Psychological Association (APA)
Hseu, You Cheng& Tsou, Hsiao-Tung& Kumar, K. J. Senthil& Lin, Kai-Yuan& Chang, Hsueh-Wei& Yang, Hsin-Ling. 2012. The Antitumor Activity of Antrodia camphorata in Melanoma Cells: Modulation of Wntβ-Catenin Signaling Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-1028165
Modern Language Association (MLA)
Hseu, You Cheng…[et al.]. The Antitumor Activity of Antrodia camphorata in Melanoma Cells: Modulation of Wntβ-Catenin Signaling Pathways. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-1028165
American Medical Association (AMA)
Hseu, You Cheng& Tsou, Hsiao-Tung& Kumar, K. J. Senthil& Lin, Kai-Yuan& Chang, Hsueh-Wei& Yang, Hsin-Ling. The Antitumor Activity of Antrodia camphorata in Melanoma Cells: Modulation of Wntβ-Catenin Signaling Pathways. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-1028165
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028165