6-Gingerol Inhibits Growth of Colon Cancer Cell LoVo via Induction of G2M Arrest
Joint Authors
Lin, Chien-Hung
Lin, Ching-Bin
Tsay, Gregory J.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
6-Gingerol, a natural component of ginger, has been widely reported to possess antiinflammatory and antitumorigenic activities.
Despite its potential efficacy against cancer, the anti-tumor mechanisms of 6-gingerol are complicated and remain sketchy.
In the present study, we aimed to investigate the anti-tumor effects of 6-gingerol on colon cancer cells.
Our results revealed that 6-gingerol treatment significantly reduced the cell viability of human colon cancer cell, LoVo, in a dose-dependent manner.
Further flow cytometric analysis showed that 6-gingerol induced significant G2/M phase arrest and had slight influence on sub-G1 phase in LoVo cells.
Therefore, levels of cyclins, cyclin-dependent kinases (CDKs), and their regulatory proteins involved in S-G2/M transition were investigated.
Our findings revealed that levels of cyclin A, cyclin B1, and CDK1 were diminished; in contrast, levels of the negative cell cycle regulators p27Kip1 and p21Cip1 were increased in response to 6-gingerol treatment.
In addition, 6-gingerol treatment elevated intracellular reactive oxygen species (ROS) and phosphorylation level of p53.
These findings indicate that exposure of 6-gingerol may induce intracellular ROS and upregulate p53, p27Kip1, and p21Cip1 levels leading to consequent decrease of CDK1, cyclin A, and cyclin B1 as result of cell cycle arrest in LoVo cells.
It would be suggested that 6-gingerol should be beneficial to treatment of colon cancer.
American Psychological Association (APA)
Lin, Ching-Bin& Lin, Chien-Hung& Tsay, Gregory J.. 2012. 6-Gingerol Inhibits Growth of Colon Cancer Cell LoVo via Induction of G2M Arrest. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1028258
Modern Language Association (MLA)
Lin, Ching-Bin…[et al.]. 6-Gingerol Inhibits Growth of Colon Cancer Cell LoVo via Induction of G2M Arrest. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-1028258
American Medical Association (AMA)
Lin, Ching-Bin& Lin, Chien-Hung& Tsay, Gregory J.. 6-Gingerol Inhibits Growth of Colon Cancer Cell LoVo via Induction of G2M Arrest. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1028258
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028258