Refined Qingkailing Protects MCAO Mice from Endoplasmic Reticulum Stress-Induced Apoptosis with a Broad Time Window
Joint Authors
Zhong, Xiang-Gen
Cheng, Fafeng
Wang, Xueqian
Lu, Yi
Guo, Shaoying
Wang, Xiaotong
Liu, Dantong
Song, Wenting
Wang, Qingguo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In the current study, we are investigating effect of refined QKL on ischemia-reperfusion-induced brain injury in mice.
Methods.
Mice were employed to induce ischemia-reperfusion injury of brain by middle cerebral artery occlusion (MCAO).
RQKL solution was administered with different doses (0, 1.5, 3, and 6 mL/kg body weight) at the same time of onset of ischemia, and with the dose of 1.5 mL/kg at different time points (0, 1.5, 3, 6, and 9 h after MCAO).
Neurological function and brain infarction were examined and cell apoptosis and ROS at prefrontal cortex were evaluated 24 h after MCAO, and western blot and intracellular calcium were also researched, respectively.
Results.
RQKL of all doses can improve neurological function and decrease brain infarction, and it performed significant effect in 0, 1.5, 3, and 6 h groups.
Moreover, RQKL was able to reduce apoptotic process by reduction of caspase-3 expression, or restraint of eIF2a phosphorylation and caspase-12 activation.
It was also able to reduce ROS and modulate intracellular calcium in the brain.
Conclusion.
RQKL can prevent ischemic-induced brain injury with a time window of 6 h, and its mechanism might be related to suppress ER stress-mediated apoptotic signaling.
American Psychological Association (APA)
Cheng, Fafeng& Zhong, Xiang-Gen& Lu, Yi& Wang, Xueqian& Song, Wenting& Guo, Shaoying…[et al.]. 2012. Refined Qingkailing Protects MCAO Mice from Endoplasmic Reticulum Stress-Induced Apoptosis with a Broad Time Window. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028397
Modern Language Association (MLA)
Cheng, Fafeng…[et al.]. Refined Qingkailing Protects MCAO Mice from Endoplasmic Reticulum Stress-Induced Apoptosis with a Broad Time Window. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-1028397
American Medical Association (AMA)
Cheng, Fafeng& Zhong, Xiang-Gen& Lu, Yi& Wang, Xueqian& Song, Wenting& Guo, Shaoying…[et al.]. Refined Qingkailing Protects MCAO Mice from Endoplasmic Reticulum Stress-Induced Apoptosis with a Broad Time Window. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028397
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028397