Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset
Joint Authors
Xu, Yu-Dong
Cui, Jian-Mei
Wei, Ying
Gao, Chang-Ke
Liu, Xiao-Yan
Shan, Chun-Xiao
Jiang, Yong-Liang
Yang, Yong Qing
Lui, Yan-Yan
Yin, Lei-Miao
Wang, Yu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-14
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Although the beneficial effects of acupuncture in asthma treatment have been well documented, little is known regarding the biological basis of this treatment.
Changes in the lung proteome of acupuncture-treated rats with asthma onset were comparatively analyzed using a two-dimensional gel electrophoresis (2DE) and mass-spectrometry- (MS-) based proteomic approach.
Acupuncture on specific acupuncture points appeared to improve respiratory function and reduce the total number of leukocytes and eosinophils in bronchoalveolar lavage fluid in OVA-induced asthma onset.
Image analysis of 2DE gels revealed 32 differentially expressed acupuncture-specific protein spots in asthma onset; 30 of which were successfully identified as 28 unique proteins using LC-MS/MS.
Bioinformatic analyses indicated that these altered proteins are most likely involved in inflammation-related biological functions, and the functional associations of these proteins result in an inflammation signaling pathway.
Acupuncture regulates the pathway at different levels by regulating several key nodal proteins, including downregulating of proinflammatory proteins (e.g., S100A8, RAGE, and S100A11) and upregulating of anti-inflammatory proteins (e.g., CC10, ANXA5, and sRAGE).
These deregulated inflammation-related proteins may mediate, at least in part, the antiasthmatic effect of acupuncture.
Further functional investigation of these acupuncture-specific effector proteins could identify new drug candidates for the prophylaxis and treatment of asthma.
American Psychological Association (APA)
Xu, Yu-Dong& Cui, Jian-Mei& Wang, Yu& Yin, Lei-Miao& Gao, Chang-Ke& Liu, Xiao-Yan…[et al.]. 2012. Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-1028583
Modern Language Association (MLA)
Xu, Yu-Dong…[et al.]. Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-1028583
American Medical Association (AMA)
Xu, Yu-Dong& Cui, Jian-Mei& Wang, Yu& Yin, Lei-Miao& Gao, Chang-Ke& Liu, Xiao-Yan…[et al.]. Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-1028583
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028583