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Inhibition of Neurotoxic Secretory Phospholipases A2 Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth
Joint Authors
Rabello, Marcelo M.
Araújo, Renata M.
Silveira, Edilberto R.
Fagundes, Fábio H. R.
Diz-Filho, Eduardo B. S.
Buzzo, Simone C.
Soares, Veronica C. G.
Toyama, Daniela de O.
Hernandes, Marcelo Z.
Monteiro, Helena S. A.
Toyama, Marcos H.
Gaeta, Henrique Hessel
Ximenes, Rafael Matos
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Secretory phospholipases A2 (sPLA2) exert proinflammatory actions through lipid mediators.
These enzymes have been found to be elevated in many inflammatory disorders such as rheumatoid arthritis, sepsis, and atherosclerosis.
The aim of this study was to evaluate the effect of harpalycin 2 (Har2), an isoflavone isolated from Harpalyce brasiliana Benth., in the enzymatic, edematogenic, and myotoxic activities of sPLA2 from Bothrops pirajai, Crotalus durissus terrificus, Apis mellifera, and Naja naja venoms.
Har2 inhibits all sPLA2 tested.
PrTX-III (B.
pirajai venom) was inhibited at about 58.7%, Cdt F15 (C.
d.
terrificus venom) at 78.8%, Apis (from bee venom) at 87.7%, and Naja (N.
naja venom) at 88.1%.
Edema induced by exogenous sPLA2 administration performed in mice paws showed significant inhibition by Har2 at the initial step.
In addition, Har2 also inhibited the myotoxic activity of these sPLA2s.
In order to understand how Har2 interacts with these enzymes, docking calculations were made, indicating that the residues His48 and Asp49 in the active site of these enzymes interacted powerfully with Har2 through hydrogen bonds.
These data pointed to a possible anti-inflammatory activity of Har2 through sPLA2 inhibition.
American Psychological Association (APA)
Ximenes, Rafael Matos& Rabello, Marcelo M.& Araújo, Renata M.& Silveira, Edilberto R.& Fagundes, Fábio H. R.& Diz-Filho, Eduardo B. S.…[et al.]. 2012. Inhibition of Neurotoxic Secretory Phospholipases A2 Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1028677
Modern Language Association (MLA)
Ximenes, Rafael Matos…[et al.]. Inhibition of Neurotoxic Secretory Phospholipases A2 Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-1028677
American Medical Association (AMA)
Ximenes, Rafael Matos& Rabello, Marcelo M.& Araújo, Renata M.& Silveira, Edilberto R.& Fagundes, Fábio H. R.& Diz-Filho, Eduardo B. S.…[et al.]. Inhibition of Neurotoxic Secretory Phospholipases A2 Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1028677
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028677