Nanobioprobe for the Determination of Pork Adulteration in Burger Formulations

Abstract EN

We report the development of a swine-specific hybrid nanobioprobe through a covalent integration of a fluorophore-labeled 27-nucleotide AluI-fragment of swine cytochrome b gene to a 3 nm gold nanoparticle for the determination of pork adulteration in processed meat products.

We tested the probe to estimate adulterated pork in ready-to-eat pork-spiked beef burgers.

The probe quantitatively detected 1–100% spiked pork in burger formulations with ≥90% accuracy.

A plot of observed fluorescence against the known concentration of AluI-digested pork DNA targets generated a concave curve, demonstrating a power relationship (y=2.956x0.509) with a regression coefficient (R2) of 0.986.

No cross-species detection was found in a standard set of pork, beef, chicken, mutton, and chevon burgers.

The method is suitable for the determination of very short-length nucleic acid targets which cannot be estimated by conventional and real-time PCR but are essential for the determination of microRNA in biodiagnostics and degraded DNA in forensic testing and food analysis.