Nanobioprobe for the Determination of Pork Adulteration in Burger Formulations
Joint Authors
Mustafa, Shuhaimi
Foo, K. L.
Hashim, U.
Ali, M. E.
Che Man, Y. B.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We report the development of a swine-specific hybrid nanobioprobe through a covalent integration of a fluorophore-labeled 27-nucleotide AluI-fragment of swine cytochrome b gene to a 3 nm gold nanoparticle for the determination of pork adulteration in processed meat products.
We tested the probe to estimate adulterated pork in ready-to-eat pork-spiked beef burgers.
The probe quantitatively detected 1–100% spiked pork in burger formulations with ≥90% accuracy.
A plot of observed fluorescence against the known concentration of AluI-digested pork DNA targets generated a concave curve, demonstrating a power relationship (y=2.956x0.509) with a regression coefficient (R2) of 0.986.
No cross-species detection was found in a standard set of pork, beef, chicken, mutton, and chevon burgers.
The method is suitable for the determination of very short-length nucleic acid targets which cannot be estimated by conventional and real-time PCR but are essential for the determination of microRNA in biodiagnostics and degraded DNA in forensic testing and food analysis.
American Psychological Association (APA)
Ali, M. E.& Mustafa, Shuhaimi& Hashim, U.& Che Man, Y. B.& Foo, K. L.. 2012. Nanobioprobe for the Determination of Pork Adulteration in Burger Formulations. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029291
Modern Language Association (MLA)
Ali, M. E.…[et al.]. Nanobioprobe for the Determination of Pork Adulteration in Burger Formulations. Journal of Nanomaterials No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-1029291
American Medical Association (AMA)
Ali, M. E.& Mustafa, Shuhaimi& Hashim, U.& Che Man, Y. B.& Foo, K. L.. Nanobioprobe for the Determination of Pork Adulteration in Burger Formulations. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029291
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029291