Cytotoxicity of Ferulic Acid on T24 Cell Line Differentiated by Different Microenvironments
Joint Authors
Chang, Chi-Huang
Chen, Kuan-Chou
Chyau, Charng-Cherng
Wang, Hui-Er
Peng, Chiung-Chi
Peng, Robert Y.
Chou, Kuang-Yu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Ferulic acid (4-hydroxy-3-methoxycinnamic acid) (FA) is a ubiquitous health beneficial phenolic acid.
Although FA has shown a diversity of biological activities including anti-inflammatory,antihypercholesterolemic and anticancer bioactivities, studies revealing its adverse effects are accumulating.
Recently, 3D-cultures are shown to exhibit uniquely biological behaviors different from that of 2D cultures.
To understand whether the cytotoxicity of FA against the T24 cell line (a bladder cancer cell line) in 2D-culture could consistently retain similar bioactivity if cultured in the 3D-systems, we conducted this experiment with 2 mM FA.
Much higher cytotoxicity was found for 3D- than 2D-culture, showing (2D vs.
3D): apoptotic rates, 64% and 76%; cell killing rates, 3.00×105 cells mmol−1·h−1 and 2.63×106 cells mmol−1·h−1, attaining a 8.77-fold.
FA upregulated the activities at 72 h (2D vs.
3D in folds that of control): SOD, 1.73-folds (P<0.05) versus 3.18 folds (P<0.001); and catalase, 2.58 versus 1.33-folds.
Comparing to the control (without FA), Bcl-2 was prominently downregulated while Bax, caspase-3 and cleaved caspase-9 were more upregulated in 3D-cultures (P<0.05).
Conclusively, different microenvironments could elicit different biological significance which in part can be ascribed to different mass transport rate.
American Psychological Association (APA)
Peng, Chiung-Chi& Chyau, Charng-Cherng& Wang, Hui-Er& Chang, Chi-Huang& Chen, Kuan-Chou& Chou, Kuang-Yu…[et al.]. 2013. Cytotoxicity of Ferulic Acid on T24 Cell Line Differentiated by Different Microenvironments. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030680
Modern Language Association (MLA)
Peng, Chiung-Chi…[et al.]. Cytotoxicity of Ferulic Acid on T24 Cell Line Differentiated by Different Microenvironments. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030680
American Medical Association (AMA)
Peng, Chiung-Chi& Chyau, Charng-Cherng& Wang, Hui-Er& Chang, Chi-Huang& Chen, Kuan-Chou& Chou, Kuang-Yu…[et al.]. Cytotoxicity of Ferulic Acid on T24 Cell Line Differentiated by Different Microenvironments. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030680
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030680