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Modulation of Pineal Melatonin Synthesis by Glutamate Involves Paracrine Interactions between Pinealocytes and Astrocytes through NF-κB Activation
Joint Authors
Villela, Darine
Atherino, Victoria Fairbanks
Lima, Larissa de Sá
Moutinho, Anderson Augusto
Peres, Rafael
Martins de Lima, Thais
Torrão, Andréa da Silva
Scavone, Cristóforo
Afeche, Solange Castro
Cipolla-Neto, Jose
Amaral, Fernanda G.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-05
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The glutamatergic modulation of melatonin synthesis is well known, along with the importance of astrocytes in mediating glutamatergic signaling in the central nervous system.
Pinealocytes and astrocytes are the main cell types in the pineal gland.
The objective of this work was to investigate the interactions between astrocytes and pinealocytes as a part of the glutamate inhibitory effect on melatonin synthesis.
Rat pinealocytes isolated or in coculture with astrocytes were incubated with glutamate in the presence of norepinephrine, and the melatonin content, was quantified.
The expression of glutamate receptors, the intracellular calcium content and the NF-κB activation were analyzed in astrocytes and pinealocytes.
TNF-α's possible mediation of the effect of glutamate was also investigated.
The results showed that glutamate's inhibitory effect on melatonin synthesis involves interactions between astrocytes and pinealocytes, possibly through the release of TNF-α.
Moreover, the activation of the astrocytic NF-κB seems to be a necessary step.
In astrocytes and pinealocytes, AMPA, NMDA, and group I metabotropic glutamate receptors were observed, as well as the intracellular calcium elevation.
In conclusion, there is evidence that the modulation of melatonin synthesis by glutamate involves paracrine interactions between pinealocytes and astrocytes through the activation of the astrocytic NF-κB transcription factor and possibly by subsequent TNF-α release.
American Psychological Association (APA)
Villela, Darine& Atherino, Victoria Fairbanks& Lima, Larissa de Sá& Moutinho, Anderson Augusto& Amaral, Fernanda G.& Peres, Rafael…[et al.]. 2013. Modulation of Pineal Melatonin Synthesis by Glutamate Involves Paracrine Interactions between Pinealocytes and Astrocytes through NF-κB Activation. BioMed Research International،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-1030722
Modern Language Association (MLA)
Villela, Darine…[et al.]. Modulation of Pineal Melatonin Synthesis by Glutamate Involves Paracrine Interactions between Pinealocytes and Astrocytes through NF-κB Activation. BioMed Research International No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-1030722
American Medical Association (AMA)
Villela, Darine& Atherino, Victoria Fairbanks& Lima, Larissa de Sá& Moutinho, Anderson Augusto& Amaral, Fernanda G.& Peres, Rafael…[et al.]. Modulation of Pineal Melatonin Synthesis by Glutamate Involves Paracrine Interactions between Pinealocytes and Astrocytes through NF-κB Activation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-1030722
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030722