Synthesis of Silica-Coated Magnetic Nanoparticles and Application in the Detection of Pathogenic Viruses
Joint Authors
Hoang Nam, Nguyen
Thi Hong, Tran
Luong, Nguyen Hoang
Quy, Dao Van
Hieu, Nguyen Minh
Tra, Pham Thi
Hai, Nguyen Hoang
Thai Son, Nguyen
Nghia, Phan Tuan
Anh, Nguyen Thi Van
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-15
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Magnetic Fe3O4 nanoparticles were prepared by coprecipitation and then coated with silica.
These Fe3O4/SiO2 nanoparticles consisted of a 10–15 nm magnetic core and a silica shell of 2–5 nm thickness.
The superparamagnetic property of the Fe3O4/SiO2 particles with the magnetization of 42.5 emu/g was confirmed by vibrating sample magnetometer (VSM).
We further optimized buffers with these Fe3O4/SiO2 nanoparticles to isolate genomic DNA of hepatitis virus type B (HBV) and of Epstein-Barr virus (EBV) for detection of the viruses based on polymerase chain reaction (PCR) amplification of a 434 bp fragment of S gene specific for HBV and 250 bp fragment of nuclear antigen encoding gene specific for EBV.
The purification efficiency of DNA of both HBV and EBV using obtained Fe3O4/SiO2 nanoparticles was superior to that obtained with commercialized Fe3O4/SiO2 microparticles, as indicated by (i) brighter PCR-amplified bands for both HBV and EBV and (ii) higher sensitivity in PCR-based detection of EBV load (copies/mL).
The time required for DNA isolation using Fe3O4/SiO2 nanoparticles was significantly reduced as the particles were attracted to magnets more quickly (15–20 s) than the commercialized microparticles (2-3 min).
American Psychological Association (APA)
Quy, Dao Van& Hieu, Nguyen Minh& Tra, Pham Thi& Hoang Nam, Nguyen& Hai, Nguyen Hoang& Thai Son, Nguyen…[et al.]. 2013. Synthesis of Silica-Coated Magnetic Nanoparticles and Application in the Detection of Pathogenic Viruses. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031464
Modern Language Association (MLA)
Quy, Dao Van…[et al.]. Synthesis of Silica-Coated Magnetic Nanoparticles and Application in the Detection of Pathogenic Viruses. Journal of Nanomaterials No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1031464
American Medical Association (AMA)
Quy, Dao Van& Hieu, Nguyen Minh& Tra, Pham Thi& Hoang Nam, Nguyen& Hai, Nguyen Hoang& Thai Son, Nguyen…[et al.]. Synthesis of Silica-Coated Magnetic Nanoparticles and Application in the Detection of Pathogenic Viruses. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031464
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031464