Osteoconductivity and Biodegradability of Collagen Scaffold Coated with Nano-β-TCP and Fibroblast Growth Factor 2

Abstract EN

Nanoparticle bioceramics have become anticipated for biomedical applications.

Highly bioactive and biodegradable scaffolds would be developed using nanoparticles of β-tricalcium phosphate (β-TCP).

We prepared collagen scaffolds coated by nano-β-TCP and fibroblast growth factor 2 (FGF2) and evaluated the effects on new bone augmentation and biodegradation.

The collagen sponge was coated with the nano-TCP dispersion and freeze-dried.

Scaffold was characterized by SEM, TEM, XRD, compressive testing and cell seeding.

Subsequently, the nano-β-TCP/collagen scaffold, collagen sponge, and each material loaded with FGF2 were implanted on rat cranial bone.

As a control, no implantation was performed.

Nano-TCP particles were found to be attached to the fibers of the collagen sponge by SEM and TEM observations.

Scaffold coated with nano-TCP showed higher compressive strength and cytocompatibility.

In histological evaluations at 10 days, inflammatory cells were rarely seen around the residual scaffold, suggesting that the nano-TCP material possesses good tissue compatibility.

At 35 days, bone augmentation and scaffold degradation in histological samples receiving nano-β-TCP scaffold were significantly greater than those in the control.

By loading of FGF2, advanced bone formation is facilitated, indicating that a combination with FGF2 would be effective for bone tissue engineering.