![](/images/graphics-bg.png)
Antimicrobial Activity of Hippurate Nanocomposite and Its Cytotoxicity Effect in Combination with Cytarabine against HL-60
Joint Authors
Ismail, Maznah
Al-Qubaisi, Mothanna
Hussein, Mohd Zobir
El Zowalaty, Mohamed E.
Hussein-Al-Ali, Samer Hasan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Hippuric acid (HA) was intercalated into a zinc-layered hydroxide (ZLH) by direct reaction of an aqueous suspension of zinc oxide with an aqueous solution of hippuric acid to obtain hippurate nanocomposite (HAN).
Various concentrations of hippuric acid (0.05, 0.2, and 0.4 molar) were used for the synthesis of the nanocomposite.
The as-synthesized HAN using 0.2 molar was found to give a well-ordered layered nanocomposite material with an increase in the basal spacing to 21.3 Å which indicated the insertion of hippurate organic moiety into the ZLH interlayers.
The cytotoxicity of HAN in combination with cytarabine against human promyelocytic leukemia cells (HL-60) was tested using MTT cell viability assay and trypan blue dye exclusion assay.
The combination of cytarabine with HAN showed higher tumor suppression efficiency as compared to that of cytarabine alone.
The IC50 values of HAN/cytarabine combination and cytarabine alone were 0.16±0.07 μg/mL and 0.17±0.09 μg/mL, respectively.
DNA fragmentation was also studied, and the exposure of HL-60 cells to cytarabine produced 10.70±0.96% DNA fragmentation compared to 18.90±1.33% when cells were exposed to combination of cytarabine with HAN.
The antimicrobial activity of hippuric acid and HAN nanocomposite was carried out against Gram-positive bacteria, Gram-negative bacteria, and yeasts.
It was found that Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus were more sensitive to HAN compared to Bacillus subtilis and Salmonella choleraesuis.
American Psychological Association (APA)
Hussein-Al-Ali, Samer Hasan& Al-Qubaisi, Mothanna& El Zowalaty, Mohamed E.& Hussein, Mohd Zobir& Ismail, Maznah. 2013. Antimicrobial Activity of Hippurate Nanocomposite and Its Cytotoxicity Effect in Combination with Cytarabine against HL-60. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031596
Modern Language Association (MLA)
Hussein-Al-Ali, Samer Hasan…[et al.]. Antimicrobial Activity of Hippurate Nanocomposite and Its Cytotoxicity Effect in Combination with Cytarabine against HL-60. Journal of Nanomaterials No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1031596
American Medical Association (AMA)
Hussein-Al-Ali, Samer Hasan& Al-Qubaisi, Mothanna& El Zowalaty, Mohamed E.& Hussein, Mohd Zobir& Ismail, Maznah. Antimicrobial Activity of Hippurate Nanocomposite and Its Cytotoxicity Effect in Combination with Cytarabine against HL-60. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031596
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031596